30S pre-rRNA is nucleolytically processed at site 1 to yield 21S pre-rRNA

Stable Identifier
Reaction [omitted]
Homo sapiens
Locations in the PathwayBrowser
SVG |   | PPTX  | SBGN
Click the image above or here to open this reaction in the Pathway Browser
The layout of this reaction may differ from that in the pathway view due to the constraints in pathway layout
An unknown endonuclease cleaves site 1 (also called site A1), removing the remainder of the 5' external transcribed spacer (5' ETS) from 30S pre-rRNA containing the 18S rRNA (Freed et al. 2012, Tomecki et al. 2015). Sixteen proteins of the small ribosomal subunit are required for processing sequences flanking the 18S rRNA in the 30S pre-rRNA (O'Donohue et al. 2010) presumably due to their assembly onto pre-rRNA as processing proceeds. Additionally, FCF1 (hUTP24, part of the SSU processome) is required for cleavage at site 1 (Tomecki et al. 2015) and NOL11 and CIRH1A (Cirhin, part of the UTPA complex) interact and are required for cleavage at site 1 and other sites (A', A0, and 2) (Freed et al. 2012). FTSJ3 and NIP7 interact and are required for processing at sites 1, 2, and A0 (Morello et al. 2011).
Literature References
PubMed ID Title Journal Year
22916032 NOL11, implicated in the pathogenesis of North American Indian childhood cirrhosis, is required for pre-rRNA transcription and processing

Prieto, JL, McStay, B, Baserga, SJ, McCann, KL, Freed, EF

PLoS Genet. 2012
20798176 The NIP7 protein is required for accurate pre-rRNA processing in human cells

Zanchin, NI, Hesling, C, Coltri, PP, Rimokh, R, Morello, LG, Castilho, BA

Nucleic Acids Res. 2011
20819938 Functional dichotomy of ribosomal proteins during the synthesis of mammalian 40S ribosomal subunits

O'Donohue, MF, Faubladier, M, Fichant, G, Choesmel, V, Gleizes, PE

J. Cell Biol. 2010
22195017 The human nucleolar protein FTSJ3 associates with NIP7 and functions in pre-rRNA processing

Zanchin, NI, Nickerson, JA, Singh, G, Silva, TC, Coltri, PP, Quaresma, AJ, Moore, MJ, Morello, LG, Oliveira, CC, Simabuco, FM

PLoS ONE 2011
26237581 hUTP24 is essential for processing of the human ribosomal RNA precursor at site A1, but not at site A0

Dziembowski, A, Tomecki, R, Labno, A, Drazkowska, K, Cysewski, D

RNA Biol 2015
This event is regulated
Orthologous Events
Cite Us!