Reactome | REG3A oligomerize to form a pore complex

REG3A oligomerize to form a pore complex

Stable Identifier

R-HSA-6801762

Type

Reaction [binding]

Species

Homo sapiens

Compartment

extracellular region

ReviewStatus

5/5

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REG3A oligomerize to form a pore complex

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Regenerating islet-derived 3A (REG3A) is thought to recognize and kill its bacterial targets in two distinct steps (Mukherjee S et al. 2014). First, REG3A is secreted from epithelial cells as a soluble monomer that recognizes Gram-positive bacteria by binding to peptidoglycan carbohydrate via an EPN motif located in the long loop region (Lehotzky RE et al. 2010). Second, REG3A kills bacteria by oligomerizing in the bacterial membrane to form a hexameric membrane-penetrating pore that is predicted to induce uncontrolled ion efflux with subsequent osmotic lysis (Mukherjee S et al. 2014). The inhibitory N-terminus of REG3A propeptide hinders lipid binding and consequently suppresses pore formation until it is removed by trypsin after secretion into the intestinal lumen (Mukherjee S et al. 2009; 2014).

Literature References

PubMed ID	Title	Journal	Year
24256734	Antibacterial membrane attack by a pore-forming intestinal C-type lectin Partch, CL, Callenberg, KM, Grabe, M, Derebe, MG, Zheng, H, Jiang, QX, Mukherjee, S, Hooper, LV, Rollins, D, Rizo, J, Propheter, DC	Nature	2014