PIAS1 SUMOylates SP3 with SUMO2

Stable Identifier
Reaction [transition]
Homo sapiens
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PIAS1 SUMOylates SP3 with SUMO2 at lysine-551 (Sapetschnig et al. 2002, Galisson et al. 2011, Tammsalu et al. 2014, Hendriks et al. 2015). SUMOylation reduces the transcription activation capacity of the long and the short isoforms of Sp3 (Sapetschnig et al 2004). Mechanistically, SUMO attachment to Sp3 serves as a molecular beacon for the recruitment of chromatin-modifying machineries that impose epigenetic silencing (inferred from Drosophila homologs in Stielow et al. 2008a, inferred from mouse homologs in Stielow et al. 2008b).

Literature References
PubMed ID Title Journal Year
12356736 Transcription factor Sp3 is silenced through SUMO modification by PIAS1

Sapetschnig, A, Rischitor, G, Braun, H, Doll, A, Schergaut, M, Melchior, F, Suske, G

EMBO J. 2002
21098080 A novel proteomics approach to identify SUMOylated proteins and their modification sites in human cells

Galisson, F, Mahrouche, L, Courcelles, M, Bonneil, E, Meloche, S, Chelbi-Alix, MK, Thibault, P

Mol. Cell Proteomics 2011
24782567 Proteome-wide identification of SUMO2 modification sites

Tammsalu, T, Matic, I, Jaffray, EG, Ibrahim, AF, Tatham, MH, Hay, RT

Sci Signal 2014
18617891 SUMO-modified Sp3 represses transcription by provoking local heterochromatic gene silencing

Stielow, B, Sapetschnig, A, Wink, C, Krüger, I, Suske, G

EMBO Rep. 2008
18374648 Identification of SUMO-dependent chromatin-associated transcriptional repression components by a genome-wide RNAi screen

Stielow, B, Sapetschnig, A, Krüger, I, Kunert, N, Brehm, A, Boutros, M, Suske, G

Mol. Cell 2008
25114211 Mapping of SUMO sites and analysis of SUMOylation changes induced by external stimuli

Impens, F, Radoshevich, L, Cossart, P, Ribet, D

Proc. Natl. Acad. Sci. U.S.A. 2014
26073453 System-wide identification of wild-type SUMO-2 conjugation sites

Hendriks, IA, D'Souza, RC, Chang, JG, Mann, M, Vertegaal, AC

Nat Commun 2015
Event Information
Go Biological Process
Catalyst Activity

SUMO transferase activity of PIAS1 [nucleoplasm]

Orthologous Events
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