CDK7 phosphorylates serine-5 and serine-7 of heptad repeats in C-terminal domain of RNA polymerase II at snRNA promoter

Stable Identifier
Reaction [transition]
Homo sapiens
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CDK7 phosphorylates serine-5 residues of heptad repeats (consensus YSPTSPS) in the C-terminal domain (CTD) of the large subunit (POLR2A) of RNA polymerase II. Serine-7 residues of the heptad repeats are also phosphorylated at promoters of snRNA genes (Egloff et al. 2007) and CDK7 is required for phosphorylation of serine-7 in vivo (Glover-Cutter et al. 2009). P-TEFb and DNA-PK are able to phosphorylate serine-7 in vitro (Glover-Cutter et al. 2009, Egloff et al. 2010). Impairment of CTD phosphorylation does not appear to affect transcription of snRNA genes but rather impairs 3' processing of the pre-snRNA (Medlin et al. 2003, Jacobs et al. 2004).
Literature References
PubMed ID Title Journal Year
19667075 TFIIH-associated Cdk7 kinase functions in phosphorylation of C-terminal domain Ser7 residues, promoter-proximal pausing, and termination by RNA polymerase II

Larochelle, S, Shokat, K, Zhang, C, Bentley, DL, Erickson, B, Fisher, RP, Glover-Cutter, K

Mol. Cell. Biol. 2009
12574128 The C-terminal domain of pol II and a DRB-sensitive kinase are required for 3' processing of U2 snRNA

Uguen, P, Murphy, S, Bentley, DL, Taylor, A, Medlin, JE

EMBO J. 2003
18079403 Serine-7 of the RNA polymerase II CTD is specifically required for snRNA gene expression

Tanzhaus, K, Chapman, RD, Murphy, S, Pitts, L, O'Reilly, D, Eick, D, Egloff, S, Taylor, A

Science 2007
14701755 Role of the C-terminal domain of RNA polymerase II in U2 snRNA transcription and 3' processing

Ogiwara, I, Weiner, AM, Jacobs, EY

Mol. Cell. Biol. 2004
20457598 The integrator complex recognizes a new double mark on the RNA polymerase II carboxyl-terminal domain

Murphy, S, Dienstbier, M, Szczepaniak, SA, Egloff, S, Taylor, A, Knight, S

J. Biol. Chem. 2010
Catalyst Activity

protein serine/threonine kinase activity of RNA polymerase II (unphosphorylated):Initiation factors at promoter of snRNA gene [nucleoplasm]

Orthologous Events
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