Reactome | Cooperation of PDCL (PhLP1) and TRiC/CCT in G-protein beta folding

Cooperation of PDCL (PhLP1) and TRiC/CCT in G-protein beta folding

Stable Identifier

R-HSA-6814122

DOI

10.3180/r-hsa-6814122.1

Type

Pathway

Species

Homo sapiens

ReviewStatus

5/5

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Cooperation of PDCL (PhLP1) and TRiC/CCT in G-protein beta folding

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The chaperonin complex TRiC/CCT is needed for the proper folding of all five G-protein beta subunits (Wells et al. 2006). TRiC/CCT cooperates with the phosducin-like protein PDCL (commonly known as PhLP or PhLP1), which interacts with both TRiC/CCT and G-protein beta subunits 1-5 (GNB1, GNB2, GNB3, GNB4, GNB5) (Dupre et al. 2007, Howlett et al. 2009). PDCL enables completion of G-protein beta folding by TRiC/CCT, promotes release of folded G-protein beta subunits 1-4 (GNB1, GNB2, GNB3, GNB4) from the chaperonin complex, and facilitates the formation of the heterodimeric G-protein beta:gamma complex between G-protein beta subunits 1-4 and G-protein gamma subunits 1-12 (Lukov et al. 2005, Lukov et al. 2006, Howlett et al. 2009, Lai et al. 2013, Plimpton et al. 2015, Xie et al. 2015). In the case of G-protein beta 5 (GNB5), PDCL stabilizes the interaction of GNB5 with the TRiC/CCT and promotes GNB5 folding, thus positively affecting formation of GNB5 dimers with RGS family proteins (Howlett et al. 2009, Lai et al. 2013, Tracy et al. 2015). However, over-expression of PDCL interferes with formation of GNB5:RGS dimers as PDCL and RGS proteins bind to the same regions of the GNB5 protein (Howlett et al. 2009).

Literature References

PubMed ID	Title	Journal	Year
17363375	Dopamine receptor-interacting protein 78 acts as a molecular chaperone for Ggamma subunits before assembly with Gbeta Mamarbachi, AM, Dupré, DJ, Richer, M, Robitaille, M, Ethier, N, Hébert, TE	J. Biol. Chem.	2007
23637185	Phosducin-like protein 1 is essential for G-protein assembly and signaling in retinal rod photoreceptors Frederick, JM, Lai, CW, Chen, CK, Baehr, W, Kolesnikov, AV, Blake, DR, Kefalov, VJ, Jiang, L, Barrow, JR, Stewart, JS, Willardson, BM	J. Neurosci.	2013
16702223	Role of the chaperonin CCT/TRiC complex in G protein betagamma-dimer assembly Dingus, J, Hildebrandt, JD, Wells, CA	J. Biol. Chem.	2006
19376773	Role of molecular chaperones in G protein beta5/regulator of G protein signaling dimer assembly and G protein betagamma dimer specificity Gray, AJ, Hunter, JM, Howlett, AC, Willardson, BM	J. Biol. Chem.	2009
16717095	Mechanism of assembly of G protein betagamma subunits by protein kinase CK2-phosphorylated phosducin-like protein and the cytosolic chaperonin complex Thulin, CD, Lukov, GL, Ludtke, PJ, Hu, T, Carter, MD, Baker, CM, Hackett, RA, Willardson, BM	J. Biol. Chem.	2006
26613416	Stable G protein-effector complexes in striatal neurons: mechanism of assembly and role in neurotransmitter signaling Martemyanov, KA, Dessauer, CW, Ueda, H, Sasaki, K, Han, PL, Lai, CW, Masuho, I, Shih, CC, Cao, Y, Xu, B, Xie, K, Willardson, BM, Ehrlich, ME	Elife	2015
15889144	Phosducin-like protein acts as a molecular chaperone for G protein betagamma dimer assembly Lukov, GL, Hu, T, Hamm, HE, McLaughlin, JN, Willardson, BM	EMBO J.	2005
25659125	Retinal cone photoreceptors require phosducin-like protein 1 for G protein complex assembly and signaling Chen, CK, Baehr, W, Kolesnikov, AV, Tracy, CM, Blake, DR, Kefalov, VJ, Willardson, BM	PLoS ONE	2015
25675501	Structures of the Gβ-CCT and PhLP1-Gβ-CCT complexes reveal a mechanism for G-protein β-subunit folding and Gβγ dimer assembly Makaju, A, Lai, CW, Prince, JT, Carrascosa, JL, Plimpton, RL, Cuellar, J, Valpuesta, JM, Aoba, T, Franklin, S, Willardson, BM, Mathis, AD	Proc. Natl. Acad. Sci. U.S.A.	2015