Reactome | Integrator complex processes the 3' end of snRNA

Integrator complex processes the 3' end of snRNA

Stable Identifier

R-HSA-6814555

Type

Reaction [omitted]

Species

Homo sapiens

Compartment

nucleoplasm

ReviewStatus

5/5

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Integrator complex processes the 3' end of snRNA

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Transcription of the pre-snRNA extends through a conserved region, the 3' box, and terminates downstream. The heterodimeric subunits INTS9 and INTS11 within the Integrator complex form an endoribonuclease that cleaves the pre-snRNA at a location 5' to the 3' box (Baillat et al. 2005, Abrecht and Wagner 2012, Skaar et al. 2015), releasing the capped snRNA bound to the cap binding complex. Factors that bind the 5' cap of the pre-snRNA enhance processing at the 3' end (Hallais et al. 2013) and polyadenlyation factors PCF11 and SKU72 are required for transcription termination (O'Reilly et al. 2014). The remainder of the transcript downstream of the cleavage site is presumably degraded by exoribonuclease.

Literature References

PubMed ID	Title	Journal	Year
22252320	snRNA 3' end formation requires heterodimeric association of integrator subunits Wagner, EJ, Albrecht, TR	Mol. Cell. Biol.	2012
24097444	Human snRNA genes use polyadenylation factors to promote efficient transcription termination Laitem, C, Murphy, S, Dienstbier, M, O'Reilly, D, Zaborowska, J, Kuznetsova, OV	Nucleic Acids Res.	2014
25675981	The Integrator complex controls the termination of transcription at diverse classes of gene targets Wu, X, Hughes, SH, Florens, LA, Saraf, A, Skaar, JR, Pagano, M, Ferris, AL, Khanna, KK, Washburn, MP	Cell Res.	2015
16239144	Integrator, a multiprotein mediator of small nuclear RNA processing, associates with the C-terminal repeat of RNA polymerase II Baillat, D, Cooch, N, Shilatifard, A, Shiekhattar, R, Näär, AM, Hakimi, MA	Cell	2005
24270878	CBC-ARS2 stimulates 3'-end maturation of multiple RNA families and favors cap-proximal processing Benbahouche, Nel H, Lener, D, Vandermoere, F, Bertrand, E, Verheggen, C, Clerici, M, Hallais, M, Gostan, T, Robert, MC, Jensen, TH, Andersen, PR, Cusack, S, Pontvianne, F	Nat. Struct. Mol. Biol.	2013

Participants

Input

RNA polymerase II (phosphoserine-2,7):RPAP2:Integrator:LEC:CBCAP:capped pre-snRNA:Initiation factors:snRNA gene [nucleoplasm] (Homo sapiens)

Output

Participates

as an event of

RNA polymerase II transcribes snRNA genes (Homo sapiens)

Catalyst Activity

RNA endonuclease activity of RNA polymerase II (phosphoserine-2,7):RPAP2:Integrator:LEC:CBCAP:capped pre-snRNA:Initiation factors:snRNA gene [nucleoplasm]

Physical Entity

RNA polymerase II (phosphoserine-2,7):RPAP2:Integrator:LEC:CBCAP:capped pre-snRNA:Initiation factors:snRNA gene [nucleoplasm] (Homo sapiens)

Activity

RNA endonuclease activity (GO:0004521)

This event is regulated

Requirements

Orthologous Events

Integrator complex processes the 3' end of snRNA (Bos taurus)

Integrator complex processes the 3' end of snRNA (Caenorhabditis elegans)

Integrator complex processes the 3' end of snRNA (Canis familiaris)

Integrator complex processes the 3' end of snRNA (Danio rerio)

Integrator complex processes the 3' end of snRNA (Dictyostelium discoideum)