Reactome | Pre-snRNA is elongated and capped with 7-methylguanosine

Pre-snRNA is elongated and capped with 7-methylguanosine

Stable Identifier

R-HSA-6814559

Type

Reaction [omitted]

Species

Homo sapiens

Compartment

nucleoplasm

ReviewStatus

5/5

Locations in the PathwayBrowser

Expand all

General

SBML | | PDF

SVG | | PPTX | SBGN

Pre-snRNA is elongated and capped with 7-methylguanosine

Click the image above or here to open this reaction in the Pathway Browser
The layout of this reaction may differ from that in the pathway view due to the constraints in pathway layout

A 7-methylguanosine triphosphate group is added to the 5' end of the pre-snRNA during transcription elongation (Mattaj 1986). The capping enzyme and cap methyltransferase involved in mRNA capping may also be responsible for this reaction. In the case of mRNA capping, the capping enzyme is targeted to the pre-mRNA by interaction with the phosphorylated C-terminal domain (CTD) of RNA polymerase II (McCracken et al. 1997). During elongation, the phosphorylation pattern of the CTD also changes: serine-5 is dephosphorylated by RPAP2 (Egloff et al. 2012) interacting with RPRD1A and RPRD1B (Ni et al. 2011, Ni et al. 2014) and serine-2 is phosphoryated by P-TEFb. Serine-7 is also phosphorylated, possibly, however the responsible kinase is not certain. The order of the capping and phosphorylation events is unknown.

Literature References

PubMed ID	Title	Journal	Year
9407024	5'-Capping enzymes are targeted to pre-mRNA by binding to the phosphorylated carboxy-terminal domain of RNA polymerase II Siderovski, D, Hessel, A, Fong, N, Bentley, DL, Foster, S, Yankulov, K, McCracken, S, Shuman, S, Rosonina, E, Brothers, G	Genes Dev.	1997
2944599	Cap trimethylation of U snRNA is cytoplasmic and dependent on U snRNP protein binding Mattaj, IW	Cell	1986
22137580	Ser7 phosphorylation of the CTD recruits the RPAP2 Ser5 phosphatase to snRNA genes Laitem, C, Murphy, S, Kiss, T, Zaborowska, J, Egloff, S	Mol. Cell	2012
24997600	RPRD1A and RPRD1B are human RNA polymerase II C-terminal domain scaffolds for Ser5 dephosphorylation Mosley, AL, Young, P, Li, J, Murphy, S, Olsen, JB, Zhong, G, He, H, Hunter, GO, Emili, A, Huang, H, Xu, C, Senisterra, GA, Min, J, Ni, Z, Kuo, WH, Guo, X, Greenblatt, JF, Loppnau, P, Arrowsmith, CH, Guo, H, Marcon, E, Tempel, W, Wan, C, El Bakkouri, M, Kuznetsova, OV, Sidhu, SS	Nat. Struct. Mol. Biol.	2014
22231121	Control of the RNA polymerase II phosphorylation state in promoter regions by CTD interaction domain-containing proteins RPRD1A and RPRD1B Guo, H, Marcon, E, Young, P, Li, J, Ruan, ED, Olsen, JB, Emili, A, Zhong, G, Ni, Z, Moffat, J, Guo, X, Greenblatt, JF	Transcription	2011

Participants

Input

Output

RNA polymerase II (phosphoserine-2,7):RPAP2:Integrator:LEC:capped pre-snRNA:Initiation factors:snRNA gene [nucleoplasm] (Homo sapiens)

Participates

as an event of

RNA polymerase II transcribes snRNA genes (Homo sapiens)

Catalyst Activity

RNA polymerase II activity of RNA polymerase II (phosphoserine-5,7):RPRD1A,B:RPRD2:RPAP2:Integrator:LEC:pre-snRNA:Initiation factors:snRNA gene [nucleoplasm]

Physical Entity

RNA polymerase II (phosphoserine-5,7):RPRD1A,B:RPRD2:RPAP2:Integrator:LEC:pre-snRNA:Initiation factors:snRNA gene [nucleoplasm] (Homo sapiens)

Activity

RNA polymerase II activity (GO:0001055)

This event is regulated

Requirements

Orthologous Events

Pre-snRNA is elongated and capped with 7-methylguanosine (Bos taurus)

Pre-snRNA is elongated and capped with 7-methylguanosine (Caenorhabditis elegans)

Pre-snRNA is elongated and capped with 7-methylguanosine (Canis familiaris)

Pre-snRNA is elongated and capped with 7-methylguanosine (Danio rerio)

Pre-snRNA is elongated and capped with 7-methylguanosine (Dictyostelium discoideum)