Reactome | DNA polymerase alpha:primase binds at the origin

DNA polymerase alpha:primase binds at the origin

Stable Identifier

R-HSA-68914

Type

Reaction [binding]

Species

Homo sapiens

Compartment

nucleoplasm

ReviewStatus

5/5

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DNA polymerase alpha:primase binds at the origin

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DNA polymerase alpha:primase is comprised of four subunits, p180, p70, p58, and p49. The two primase subunits, p58 and p49, form a tight complex. The p49 subunit contains the DNA primase activity and one role of p58 appears to be tethering p49 to p180, the DNA polymerase catalytic subunit. The fourth subunit, p70, binds p180 and may tether the DNA polymerase alpha:primase complex to Cdc45.

Literature References

PubMed ID	Title	Journal	Year
12045100	DNA replication in eukaryotic cells. Bell, SP, Dutta, A	Annu Rev Biochem	2002
10882098	Initiation of eukaryotic DNA replication: origin unwinding and sequential chromatin association of Cdc45, RPA, and DNA polymerase alpha. Newport, J, Walter, J	Mol Cell	2000
10757793	Assembly of a complex containing Cdc45p, replication protein A, and Mcm2p at replication origins controlled by S-phase cyclin-dependent kinases and Cdc7p-Dbf4p kinase. Stillman, B, Zou, L	Mol Cell Biol	2000