glutamine + H2O => glutamate + NH4+ [GLS]

Stable Identifier
Reaction [transition]
Homo sapiens
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Mitochondrial glutaminase (GLS) catalyzes the hydrolysis of glutamine to yield glutamate and ammonia. Two GLS enzymes have been identified, one abundantly expressed in the liver (GLS - Elgadi et al. 1999) and one abundantly expressed in kidney (GLS2 - Gomez-Fabre et al. 2000). Their biochemical properties are similar. The enzymes are inferred to function as dimers based on unpublished crystallographic data for GLS (PDB 3CZD) and studies of glutaminase enzyme purified from Ehrlich Ascites cells (Quesada et al. 1988).
Literature References
PubMed ID Title Journal Year
10620514 Molecular cloning, sequencing and expression studies of the human breast cancer cell glutaminase

Aledo, JC, Alonso, FJ, Gomez-Fabre, PM, Nunez De Castro, I, Marquez, J, Campos, JA, Del Castillo-Olivares, A

Biochem J 2000
3214421 Purification of phosphate-dependent glutaminase from isolated mitochondria of Ehrlich ascites-tumour cells

Medina, MA, Nunez De Castro, I, Marquez, J, Quesada, AR, Sánchez-Jiménez, F, Perez-Rodriguez, J

Biochem J 1988
11015561 Cloning and analysis of unique human glutaminase isoforms generated by tissue-specific alternative splicing

Souba, WW, Elgadi, KM, Qian, M, Meguid, RA, Abcouwer, SF

Physiol Genomics 1999
Catalyst Activity

glutaminase activity of GLS dimers [mitochondrial matrix]

Orthologous Events
Cross References
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