Addition of the third nucleotide on the nascent transcript

Stable Identifier
R-HSA-75850
Type
Reaction [omitted]
Species
Homo sapiens
Compartment
ReviewStatus
5/5
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Formation of the second phosphodiester bond creates a 3-nt product. This short transcript is still loosely associated with the RNA polymerase II initiation complex and can dissociate to yield abortive products, which are not further extended. The transcription complex still requires continued ATP-hydrolysis by TFIIH and remains sensitive to single-stranded oligo-nucleotide inhibition.

The open region (“transcription bubble”) expands concomitant with the site of RNA-extension. In this case this region spans positions -9 to +3.

Literature References
PubMed ID Title Journal Year
11784853 Translocation after synthesis of a four-nucleotide RNA commits RNA polymerase II to promoter escape.

Goodrich, JA, Kugel, JF

Mol Cell Biol 2002
9405375 Three transitions in the RNA polymerase II transcription complex during initiation.

Fiedler, U, Timmers, HT, Holstege, FC

EMBO J 1998
Participants
Participates
Catalyst Activity

DNA-directed 5'-3' RNA polymerase activity of RNA Polymerase II (unphosphorylated):TFIIF complex [nucleoplasm]

Orthologous Events
Authored
Created
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