Addition of the third nucleotide on the nascent transcript

Stable Identifier
R-HSA-75850
Type
Reaction [omitted]
Species
Homo sapiens
Compartment
nucleoplasm
ReviewStatus
5/5
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Addition of the third nucleotide on the nascent transcript
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Formation of the second phosphodiester bond creates a 3-nt product. This short transcript is still loosely associated with the RNA polymerase II initiation complex and can dissociate to yield abortive products, which are not further extended. The transcription complex still requires continued ATP-hydrolysis by TFIIH and remains sensitive to single-stranded oligo-nucleotide inhibition.

The open region (“transcription bubble”) expands concomitant with the site of RNA-extension. In this case this region spans positions -9 to +3.

Literature References
PubMed ID Title Journal Year
11784853 Translocation after synthesis of a four-nucleotide RNA commits RNA polymerase II to promoter escape.

Kugel, JF, Goodrich, JA

Mol Cell Biol 2002
9405375 Three transitions in the RNA polymerase II transcription complex during initiation.

Holstege, FC, Fiedler, U, Timmers, HT

EMBO J 1998
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Catalyst Activity

DNA-directed RNA polymerase activity of RNA Polymerase II (unphosphorylated):TFIIF complex [nucleoplasm]

Physical Entity
RNA Polymerase II (unphosphorylated):TFIIF complex [nucleoplasm] (Homo sapiens)
Activity
DNA-directed RNA polymerase activity (GO:0003899)
Orthologous Events
Addition of the third nucleotide on the nascent HIV-1 transcript (Homo sapiens)
Addition of the third nucleotide on the nascent transcript (Bos taurus)
Addition of the third nucleotide on the nascent transcript (Caenorhabditis elegans)
Addition of the third nucleotide on the nascent transcript (Canis familiaris)
Addition of the third nucleotide on the nascent transcript (Danio rerio)
Addition of the third nucleotide on the nascent transcript (Dictyostelium discoideum)
Addition of the third nucleotide on the nascent transcript (Drosophila melanogaster)
Addition of the third nucleotide on the nascent transcript (Mus musculus)
Addition of the third nucleotide on the nascent transcript (Rattus norvegicus)
Addition of the third nucleotide on the nascent transcript (Saccharomyces cerevisiae)
Addition of the third nucleotide on the nascent transcript (Schizosaccharomyces pombe)
Addition of the third nucleotide on the nascent transcript (Sus scrofa)
Addition of the third nucleotide on the nascent transcript (Xenopus tropicalis)
Authored
Timmers, HTM  (2003-09-11)
Created
Timmers, HTM  (2003-09-11)
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