TREX complex binds spliced, capped mRNA:CBC:EJC cotranscriptionally

Stable Identifier
Reaction [binding]
Homo sapiens
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The THO subcomplex of the TREX complex initially interacts with the serine-2,5 phosphorylated C-terminal domain of RNA polymerase II (Strasser et al. 2002, Inferred from yeast in Meinel et al. 2013) then with CBP80 of the cap binding complex (Cheng et al. 2006, Dufu et al. 2010, Chi et al. 2013). A TREX complex binds spliced mRNA near the cap during transcription (Cheng et al. 2006). Recruitment is dependent on splicing of the mRNA (Masuda et al. 2005). THO/TREX is required for efficient mRNP biogenesis and export (reviewed in Luna et al 2012). In yeast, components of the THO/TREX complex also affect transcription and 3' processing of mRNA, (Rondon et al. 2003, Rougemaille et al. 2008, Johnson et al. 2011, reviewed in Katahira 2015), however the human TREX complex does not appear to affect transcription (Masuda et al. 2005). The AREX complex, which contains DDX39A (UHR49) rather than DDX39B (UAP56) appears to perform the same function as TREX in mRNA export, but acts on a different subset of mRNAs (Yamazaki et al. 2010).

Literature References
PubMed ID Title Journal Year
18957205 THO/Sub2p functions to coordinate 3'-end processing with gene-nuclear pore association

Rougemaille, M, Dieppois, G, Kisseleva-Romanova, E, Gudipati, RK, Lemoine, S, Blugeon, C, Boulay, J, Jensen, TH, Stutz, F, Devaux, F, Libri, D

Cell 2008
21947206 The export factor Yra1 modulates mRNA 3' end processing

Johnson, SA, Kim, H, Erickson, B, Bentley, DL

Nat. Struct. Mol. Biol. 2011
15998806 Recruitment of the human TREX complex to mRNA during splicing

Masuda, S, Das, R, Cheng, H, Hurt, E, Dorman, N, Reed, R

Genes Dev. 2005
12871933 Molecular evidence that the eukaryotic THO/TREX complex is required for efficient transcription elongation

Rondón, AG, Jimeno, S, García-Rubio, M, Aguilera, A

J. Biol. Chem. 2003
22207203 New clues to understand the role of THO and other functionally related factors in mRNP biogenesis

Luna, R, Rondón, AG, Aguilera, A

Biochim. Biophys. Acta 2012
22928037 The proteins PDIP3 and ZC11A associate with the human TREX complex in an ATP-dependent manner and function in mRNA export

Folco, EG, Lee, CS, Dufu, K, Yamazaki, T, Reed, R

PLoS ONE 2012
23299939 Chtop is a component of the dynamic TREX mRNA export complex

Chang, CT, Hautbergue, GM, Walsh, MJ, Viphakone, N, van Dijk, TB, Philipsen, S, Wilson, SA

EMBO J. 2013
23222130 Aly and THO are required for assembly of the human TREX complex and association of TREX components with the spliced mRNA

Chi, B, Wang, Q, Wu, G, Tan, M, Wang, L, Shi, M, Chang, X, Cheng, H

Nucleic Acids Res. 2013
25836925 Nuclear export of messenger RNA

Katahira, J

Genes (Basel) 2015
19836239 UIF, a New mRNA export adaptor that works together with REF/ALY, requires FACT for recruitment to mRNA

Hautbergue, GM, Hung, ML, Walsh, MJ, Snijders, AP, Chang, CT, Jones, R, Ponting, CP, Dickman, MJ, Wilson, SA

Curr. Biol. 2009
17190602 Human mRNA export machinery recruited to the 5' end of mRNA

Cheng, H, Dufu, K, Lee, CS, Hsu, JL, Dias, A, Reed, R

Cell 2006
20844015 ATP is required for interactions between UAP56 and two conserved mRNA export proteins, Aly and CIP29, to assemble the TREX complex

Dufu, K, Livingstone, MJ, Seebacher, J, Gygi, SP, Wilson, SA, Reed, R

Genes Dev. 2010
25662211 Luzp4 defines a new mRNA export pathway in cancer cells

Viphakone, N, Cumberbatch, MG, Livingstone, MJ, Heath, PR, Dickman, MJ, Catto, JW, Wilson, SA

Nucleic Acids Res. 2015
24244187 Recruitment of TREX to the transcription machinery by its direct binding to the phospho-CTD of RNA polymerase II

Meinel, DM, Burkert-Kautzsch, C, Kieser, A, O'Duibhir, E, Siebert, M, Mayer, A, Cramer, P, Söding, J, Holstege, FC, Strässer, K

PLoS Genet. 2013
20573985 The closely related RNA helicases, UAP56 and URH49, preferentially form distinct mRNA export machineries and coordinately regulate mitotic progression

Yamazaki, T, Fujiwara, N, Yukinaga, H, Ebisuya, M, Shiki, T, Kurihara, T, Kioka, N, Kambe, T, Nagao, M, Nishida, E, Masuda, S

Mol. Biol. Cell 2010
17984224 ATP-dependent recruitment of export factor Aly/REF onto intronless mRNAs by RNA helicase UAP56

Taniguchi, I, Ohno, M

Mol. Cell. Biol. 2008
18411249 Binding of ATP to UAP56 is necessary for mRNA export

Kota, KP, Wagner, SR, Huerta, E, Underwood, JM, Nickerson, JA

J. Cell. Sci. 2008
11979277 TREX is a conserved complex coupling transcription with messenger RNA export

Strässer, K, Masuda, S, Mason, P, Pfannstiel, J, Oppizzi, M, Rodriguez-Navarro, S, Rondón, AG, Aguilera, A, Struhl, K, Reed, R, Hurt, E

Nature 2002
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