Reactome | Clathrin recruits auxilins to the clathrin-coated vesicle

Clathrin recruits auxilins to the clathrin-coated vesicle

Stable Identifier

R-HSA-8868659

Type

Reaction [binding]

Species

Homo sapiens

Compartment

clathrin-coated endocytic vesicle membrane

ReviewStatus

5/5

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Clathrin recruits auxilins to the clathrin-coated vesicle

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After fission from the plasma membrane, auxilin proteins DNAJC6 and GAK are recruited to the vesicle through interaction with clathrin and phosphoinositides, in particular PI4P (Greener et al, 2000; Lee et al, 2006; Massol et al, 2006; Taylor et al, 2011; Scheele et al, 2001; Fotin et al, 2004a; Fotin et al, 2004b; Guan et al, 2010; reviewed in McMahon and Boucrot, 2011; Sousa and Lafer, 2015). Auxilin in turn recruits the ATPase HSPA8 (also known as HSC70) , which uses the energy from ATP hydrolysis to remove the clathrin-coat from the vesicle, priming it for fusion with a subsequent endosomal compartment (Schlossman et al, 1984; Ungewickell et al, 1995; Rappoport et al, 2008; Xing et al, 2010; Bocking et al, 2011; Rothnie et al, 2011; reviewed in McMahon and Boucrot, 2011; Sousa and Lafer, 2015).

Literature References

PubMed ID	Title	Journal	Year
21779028	Molecular mechanism and physiological functions of clathrin-mediated endocytosis McMahon, HT, Boucrot, E	Nat. Rev. Mol. Cell Biol.	2011
10625686	Role of cyclin G-associated kinase in uncoating clathrin-coated vesicles from non-neuronal cells Greener, T, Zhao, X, Nojima, H, Eisenberg, E, Greene, LE	J. Biol. Chem.	2000
6146630	An enzyme that removes clathrin coats: purification of an uncoating ATPase Schlossman, DM, Schmid, SL, Braell, WA, Rothman, JE	J. Cell Biol.	1984
26042225	The role of molecular chaperones in clathrin mediated vesicular trafficking Sousa, R, Lafer, EM	Front Mol Biosci	2015
8524399	Role of auxilin in uncoating clathrin-coated vesicles Ungewickell, E, Ungewickell, H, Holstein, SE, Lindner, R, Prasad, K, Barouch, W, Martin, B, Greene, LE, Eisenberg, E	Nature	1995
17978091	A motif in the clathrin heavy chain required for the Hsc70/auxilin uncoating reaction Rapoport, I, Boll, W, Yu, A, Böcking, T, Kirchhausen, T	Mol. Biol. Cell	2008
20826345	Structure of the PTEN-like region of auxilin, a detector of clathrin-coated vesicle budding Guan, R, Dai, H, Han, D, Harrison, SC, Kirchhausen, T	Structure	2010
15502813	Structure of an auxilin-bound clathrin coat and its implications for the mechanism of uncoating Fotin, A, Cheng, Y, Grigorieff, N, Walz, T, Harrison, SC, Kirchhausen, T	Nature	2004
21445324	A high precision survey of the molecular dynamics of mammalian clathrin-mediated endocytosis Taylor, MJ, Perrais, D, Merrifield, CJ	PLoS Biol.	2011
20033059	Structure of clathrin coat with bound Hsc70 and auxilin: mechanism of Hsc70-facilitated disassembly Xing, Y, Böcking, T, Wolf, M, Grigorieff, N, Kirchhausen, T, Harrison, SC	EMBO J.	2010
15502812	Molecular model for a complete clathrin lattice from electron cryomicroscopy Fotin, A, Cheng, Y, Sliz, P, Grigorieff, N, Harrison, SC, Kirchhausen, T, Walz, T	Nature	2004
16798879	A burst of auxilin recruitment determines the onset of clathrin-coated vesicle uncoating Massol, RH, Boll, W, Griffin, AM, Kirchhausen, T	Proc. Natl. Acad. Sci. U.S.A.	2006
11470803	Multiple interactions of auxilin 1 with clathrin and the AP-2 adaptor complex Scheele, U, Kalthoff, C, Ungewickell, E	J. Biol. Chem.	2001