Reactome | Auxilin recruits HSPA8:ATP to the clathrin-coated vesicle

Auxilin recruits HSPA8:ATP to the clathrin-coated vesicle

Stable Identifier

R-HSA-8868660

Type

Reaction [binding]

Species

Homo sapiens

Compartment

clathrin-coated endocytic vesicle membrane

ReviewStatus

5/5

Locations in the PathwayBrowser

Expand all

General

SBML | | PDF

SVG | | PPTX | SBGN

Auxilin recruits HSPA8:ATP to the clathrin-coated vesicle

Click the image above or here to open this reaction in the Pathway Browser
The layout of this reaction may differ from that in the pathway view due to the constraints in pathway layout

HSPA8 (also known as HSC70) is recruited to the clathrin-coated vesicle through interaction with DNA J proteins GAK and DNAJC6 (Rapoport et al, 2008; Xing et al, 2010; reviewed in Sousa and Lafer, 2015). Recent studies examining the stoichiometry of uncoating predict between one and three HSPA8 molecules are required per clathrin triskelion for maximal uncoating in vitro (Bocking et al, 2011; Rothnie et al, 2011). After ATP hydrolysis, HSPA8 remains associated with the liberated clathrin, which prevents aberrant repolymerization and association of clathrin (Schlossman et al, 1984; reviewed in Sousa and Lafer, 2015).

Literature References

PubMed ID	Title	Journal	Year
17978091	A motif in the clathrin heavy chain required for the Hsc70/auxilin uncoating reaction Rapoport, I, Boll, W, Yu, A, Böcking, T, Kirchhausen, T	Mol. Biol. Cell	2008
6146630	An enzyme that removes clathrin coats: purification of an uncoating ATPase Schlossman, DM, Schmid, SL, Braell, WA, Rothman, JE	J. Cell Biol.	1984
21278753	Single-molecule analysis of a molecular disassemblase reveals the mechanism of Hsc70-driven clathrin uncoating Böcking, T, Aguet, F, Harrison, SC, Kirchhausen, T	Nat. Struct. Mol. Biol.	2011
26042225	The role of molecular chaperones in clathrin mediated vesicular trafficking Sousa, R, Lafer, EM	Front Mol Biosci	2015
21482805	A sequential mechanism for clathrin cage disassembly by 70-kDa heat-shock cognate protein (Hsc70) and auxilin Rothnie, A, Clarke, AR, Kuzmic, P, Cameron, A, Smith, CJ	Proc. Natl. Acad. Sci. U.S.A.	2011
20033059	Structure of clathrin coat with bound Hsc70 and auxilin: mechanism of Hsc70-facilitated disassembly Xing, Y, Böcking, T, Wolf, M, Grigorieff, N, Kirchhausen, T, Harrison, SC	EMBO J.	2010