Dynamin-mediated GTP hydrolysis promotes vesicle scission

Stable Identifier
Reaction [transition]
Homo sapiens
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Self-assembly of dynamin around the neck of the emerging clathrin-coated vesicle stimulates its GTPase activity. This in turn promotes a conformational change in dynamin organization that is required for membrane fission (Hinshaw and Schmid, 1995; Sweitzer and Hinshaw, 1998; Takei et al, 1999; Yoshida et al, 2004; Chappie et al, 2010; Chappie et al, 2011; Ford et al, 2011; Faelber et al, 2011; reviewed in Daumke et al, 2014).
Literature References
PubMed ID Title Journal Year
10559861 Functional partnership between amphiphysin and dynamin in clathrin-mediated endocytosis

Slepnev, VI, Haucke, V, Takei, K, De Camilli, P

Nat. Cell Biol. 1999
21927001 The crystal structure of dynamin

Nunnari, J, Jenni, S, Ford, MG

Nature 2011
21962517 A pseudoatomic model of the dynamin polymer identifies a hydrolysis-dependent powerstroke

Fang, S, Leonard, M, Mears, JA, Milligan, RA, Chappie, JS, Dyda, F, Hinshaw, JE, Schmid, SL

Cell 2011
24581490 BAR domain scaffolds in dynamin-mediated membrane fission

Daumke, O, Haucke, V, Roux, A

Cell 2014
21927000 Crystal structure of nucleotide-free dynamin

NoƩ, F, Held, M, Posor, Y, Daumke, O, Haucke, V, Schulze, D, Faelber, K, Roske, Y, Gao, S

Nature 2011
15318165 The stimulatory action of amphiphysin on dynamin function is dependent on lipid bilayer curvature

Cremona, O, Takei, K, Araki, K, Abe, T, Yoshida, Y, Yasuda, T, Di Paolo, G, Kinuta, M, Liang, S, De Camilli, P, Moriyama, Y

EMBO J. 2004
20428113 G domain dimerization controls dynamin's assembly-stimulated GTPase activity

Leonard, M, Acharya, S, Chappie, JS, Dyda, F, Schmid, SL

Nature 2010
9635431 Dynamin undergoes a GTP-dependent conformational change causing vesiculation

Hinshaw, JE, Sweitzer, SM

Cell 1998
7877694 Dynamin self-assembles into rings suggesting a mechanism for coated vesicle budding

Hinshaw, JE, Schmid, SL

Nature 1995
Catalyst Activity

GTPase activity of PI(4)P:p-T156 AP-2:clathrin:ITSNs:EPS15:REPS1:SGIP1:NECAPs:AAK1:CLASP proteins:cargo:F-BAR proteins:BAR domain proteins:ARP2/3 complex:WASL:f-actin:HIP dimers:DNM:GTP:SYNJs [plasma membrane]

Orthologous Events
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