Reactome | RAB9 GEFs exchange GTP for GDP on RAB9

RAB9 GEFs exchange GTP for GDP on RAB9

Stable Identifier

R-HSA-8876191

Type

Reaction [transition]

Species

Homo sapiens

Compartment

trans-Golgi network membrane, cytosol

ReviewStatus

5/5

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RAB9 plays a role in the retrograde traffic of cargo such as the mannose-6-phosphate receptors (M6PR) from the late endosome to the trans-Golgi network (TGN). Vesicles are recruited to the TGN through interaction of RAB9 with the atypical RHO GTPase RHOBTB3, and tethered by virtue of interaction with TGN-localized Golgins and the GARP complex (Perez-Victoria et al, 2008; Perez-Victoria et al, 2009; Diaz et al, 1999; Espinosa et al, 2009; reviewed in Pfeffer, 2011; Chia and Gleeson, 2014). Interaction of RAB9:GDP with its GEFs promotes release of GDP, allowing GTP to bind, and precludes the interaction of RAB9 with GDI and CHM proteins. DENND2 family members have been shown to be RAB9 specific GEFs, and HeLa cells depleted of RAB9 or DENND2A show reduced staining of M6PR and a loss of M6PR-positive structures in the cell periphery (Yoshimura et al, 2010).

Literature References

PubMed ID	Title	Journal	Year
18367545	Requirement of the human GARP complex for mannose 6-phosphate-receptor-dependent sorting of cathepsin D to lysosomes Mardones, GA, Pérez-Victoria, FJ, Bonifacino, JS	Mol. Biol. Cell	2008
20937701	Family-wide characterization of the DENN domain Rab GDP-GTP exchange factors Linford, A, Rigden, DJ, Barr, FA, Gerondopoulos, A, Yoshimura, S	J. Cell Biol.	2010
25343031	Membrane tethering Chia, PZ, Gleeson, PA	F1000Prime Rep	2014
19620288	Dual roles of the mammalian GARP complex in tethering and SNARE complex assembly at the trans-golgi network Pérez-Victoria, FJ, Bonifacino, JS	Mol. Cell. Biol.	2009
25341920	Rab proteins and the compartmentalization of the endosomal system Wandinger-Ness, A, Zerial, M	Cold Spring Harb Perspect Biol	2014
21421921	Entry at the trans-face of the Golgi Pfeffer, SR	Cold Spring Harb Perspect Biol	2011
9590177	TIP47: a cargo selection device for mannose 6-phosphate receptor trafficking Pfeffer, SR, Díaz, E	Cell	1998
27246931	Multiple Types of Guanine Nucleotide Exchange Factors (GEFs) for Rab Small GTPases Oguchi, ME, Fukuda, M, Ishida, M	Cell Struct. Funct.	2016
19490898	RhoBTB3: a Rho GTPase-family ATPase required for endosome to Golgi transport Espinosa, EJ, Calero, M, Sridevi, K, Pfeffer, SR	Cell	2009