Reactome | MADD exchanges GTP for GDP on RAB27

MADD exchanges GTP for GDP on RAB27

Stable Identifier

R-HSA-8877308

Type

Reaction [transition]

Species

Homo sapiens

Compartment

melanosome membrane, cytosol

ReviewStatus

5/5

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Vertebrate RAB27 exists in two isoforms, RAB27A and RAB27B, with overlapping but distinct functions in trafficking to melanosomes, secretory granules and platelets (Kondo et al, 2006; Figueiredo et al, 2008; Tarafder et al, 2011; reviewed in Fukuda et al, 2013). Studies in nematodes and vertebrates have identified MADD as a RAB27 GEF required for its activity (Mahoney et al, 2006; Tarafder et al, 2011; Yoshimura et al, 2010). Interaction of RAB27:GDP with its GEFs promotes release of GDP, allowing GTP to bind, and precludes the interaction of RAB27 with GDI and CHM proteins.

Literature References

PubMed ID	Title	Journal	Year
21554507	Rab27a targeting to melanosomes requires nucleotide exchange but not effector binding Seabra, MC, Wasmeier, C, Figueiredo, AC, Tarafder, AK, Ramalho, JS, Orihara, A, Hume, AN, Booth, AE	Traffic	2011
18559336	Rab3GEP is the non-redundant guanine nucleotide exchange factor for Rab27a in melanocytes Seabra, MC, Wasmeier, C, Baron, RA, Figueiredo, AC, Tarafder, AK, Ramalho, JS	J. Biol. Chem.	2008
16880209	Constitutive GDP/GTP exchange and secretion-dependent GTP hydrolysis activity for Rab27 in platelets Fukuda, M, Kondo, H, Horiuchi, H, Shirakawa, R, Higashi, T, Kita, T, Kawato, M	J. Biol. Chem.	2006
20937701	Family-wide characterization of the DENN domain Rab GDP-GTP exchange factors Linford, A, Rigden, DJ, Barr, FA, Gerondopoulos, A, Yoshimura, S	J. Cell Biol.	2010
16571673	Regulation of synaptic transmission by RAB-3 and RAB-27 in Caenorhabditis elegans Fukuda, M, Wang, ZW, Itoh, T, Nonet, ML, Hadwiger, G, Mahoney, TR, Luo, S, Vincent, R, Liu, Q	Mol. Biol. Cell	2006
23678941	Rab27 effectors, pleiotropic regulators in secretory pathways Fukuda, M	Traffic	2013