Protein serine/threonine kinase HIPK2 phosphorylates RUNX1 upon formation of the RUNX1:CBFB complex. Serine residues S249 and S273 and threonine residue S276 of RUNX1 are phosphorylated by HIPK2. HIPK2-mediated phosphorylation of RUNX1 is implicated as an important regulatory step in hematopoiesis and is disrupted by leukemogenic mutations in CBFB (Aikawa et al. 2006, Wee et al. 2008).
protein serine/threonine kinase activity of HIPK2 [nucleoplasm]