Transfer of NEDD8 to AcM-UBE2F

Stable Identifier
R-HSA-8951764
Type
Reaction [transition]
Species
Homo sapiens
Compartment
ReviewStatus
5/5
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UBA3:NAE1 transfers NEDD8 to the catalytic cysteine residue of UBE2F. Because two of the three E1-E2 interaction interfaces are created by conformational states present in the doubly-NEDDylated NAE1, transfer of NEDD8 to UBE2M is thought to weaken the interaction between UBA3:NAE1 and UBE2F, contributing to UBE2F release (Walden et al, 2003; Huang et al, 2004; Hunag et al, 2005; Huang et al, 2007).
Literature References
PubMed ID Title Journal Year
14690597 The structure of the APPBP1-UBA3-NEDD8-ATP complex reveals the basis for selective ubiquitin-like protein activation by an E1

Miller, DW, Huang, DT, Howard, RJ, Schulman, BA, Holton, JM, Minor, DL, Walden, H, Podgorski, MS

Mol. Cell 2003
17220875 Basis for a ubiquitin-like protein thioester switch toggling E1-E2 affinity

Huang, DT, Ohi, MD, Hunt, HW, Schulman, BA, Holton, JM, Zhuang, M

Nature 2007
15694336 Structural basis for recruitment of Ubc12 by an E2 binding domain in NEDD8's E1

Huang, DT, Schulman, BA, Holton, JM, Paydar, A, Waddell, MB, Zhuang, M

Mol. Cell 2005
15361859 A unique E1-E2 interaction required for optimal conjugation of the ubiquitin-like protein NEDD8

Miller, DW, Huang, DT, Cassell, R, Schulman, BA, Roussel, MF, Holton, JM, Mathew, R

Nat. Struct. Mol. Biol. 2004
Participants
Participates
Catalyst Activity

NEDD8 transferase activity of AcM-UBE2F:NEDD8-UBA3:NAE1:NEDD8 [cytosol]

Orthologous Events
Authored
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