Reactome | Doubly neddylated UBA3:NAE1 binds AcM-UBE2F

Doubly neddylated UBA3:NAE1 binds AcM-UBE2F

Stable Identifier

R-HSA-8951766

Type

Reaction [binding]

Species

Homo sapiens

Compartment

cytosol

ReviewStatus

5/5

Locations in the PathwayBrowser

Expand all

General

SBML | | PDF

SVG | | PPTX | SBGN

Doubly neddylated UBA3:NAE1 binds AcM-UBE2F

Click the image above or here to open this reaction in the Pathway Browser
The layout of this reaction may differ from that in the pathway view due to the constraints in pathway layout

When NAE1:UBA3 is doubly loaded with NEDD8 (one molecule covalently attached to the catalytic cysteine and the other bound in the adenylation site), the E1 enzyme is competent to interact with either of its E2 enzymes, UBE2F and UBE2M (also known as UBC12). Three binding interfaces contribute to the interaction of the E1 and E2 enzymes. When doubly neddylated, the ubiquitin fold domain of NAE1 reorients and, in conjunction with the adenylation domain, forms a cryptic E2-binding site. The adenylation domain also makes contact with the amino terminus of either E2 enzyme. UBE2M additionally interacts directly with the NEDD8 molecule covalently attached to the E1 catalytic cysteine (Huang et al, 2004; Huang et al, 2007; reviewed in Enchev et al, 2015).
UBE2F is the E2 responsible for the transfer of NEDD8 to the CUL5:RBX2 E3 ligase complex, while UBE2M is specific for RBX1-containing E3 ligase complexes formed with CUL1-4 (Huang et al, 2009; reviewed in Mahon et al, 2014).

Literature References

PubMed ID	Title	Journal	Year
25314029	Cullin E3 ligases and their rewiring by viral factors Pick, E, Mahon, C, Krogan, NJ, Craik, CS	Biomolecules	2014
17220875	Basis for a ubiquitin-like protein thioester switch toggling E1-E2 affinity Huang, DT, Ohi, MD, Hunt, HW, Schulman, BA, Holton, JM, Zhuang, M	Nature	2007
15361859	A unique E1-E2 interaction required for optimal conjugation of the ubiquitin-like protein NEDD8 Miller, DW, Huang, DT, Cassell, R, Schulman, BA, Roussel, MF, Holton, JM, Mathew, R	Nat. Struct. Mol. Biol.	2004
25531226	Protein neddylation: beyond cullin-RING ligases Schulman, BA, Enchev, RI, Peter, M	Nat. Rev. Mol. Cell Biol.	2015
19250909	E2-RING expansion of the NEDD8 cascade confers specificity to cullin modification Borg, LA, Huang, DT, Duda, DM, Hunt, HW, Murray, PJ, Schulman, BA, Roussel, MF, Neale, G, Ayrault, O, Taherbhoy, AM, Scott, DC	Mol. Cell	2009