Calcium-dependent protein kinases, PKA and CaMK IV, activated by neuronal membrane depolarization, phosphorylate MECP2 at threonine residue T308. Only the PKA C-alpha isoform (PRKACA) was experimentally tested. T308 corresponds to T320 in the longer MECP2 splicing isoform, MECP2_e1 (MECP2B). Phosphorylation of MECP2 at threonine residue T308 is correlated with neuronal activity and inhibits binding of MECP2 to the nuclear receptor co-repressor complex (NCoR/SMRT) (Ebert et al. 2013).
calcium-dependent protein kinase activity of Active PRKACA,CaMK IV [nucleoplasm]