Reactome | HRSP12 deaminates 2-iminobutanoate to 2OBUTA

HRSP12 deaminates 2-iminobutanoate to 2OBUTA

Stable Identifier

R-HSA-9014641

Type

Reaction [transition]

Species

Homo sapiens

Compartment

cytosol

Synonyms

2-iminobutanoate + H2O => 2-oxobutanoate + NH4(+)

ReviewStatus

5/5

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HRSP12 deaminates 2-iminobutanoate to 2OBUTA

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The toxic enamine/imine intermediates generated by pyridoxal 5'-phosphate (PXLP) containing enzymes can cause severe cellular damage if allowed to accumulate (Downs & Ernst 2015). 2-iminobutanoate/2-iminopropanoate deaminase (RIDA aka HRSP12) is a widely conserved protein that prevents 2-iminobutanoate accumulation by facilitating its conversion to the stable metabolite 2-oxobutanoate (2OBUTA aka 2-ketobutyrate) (Cooper et al. 2011, Lambrecht et al. 2012, 2013, Niehaus et al. 2015).

Literature References

PubMed ID	Title	Journal	Year
22094463	Conserved YjgF protein family deaminates reactive enamine/imine intermediates of pyridoxal 5'-phosphate (PLP)-dependent enzyme reactions Lambrecht, JA, Flynn, JM, Downs, DM	J. Biol. Chem.	2012
23386433	RidA proteins prevent metabolic damage inflicted by PLP-dependent dehydratases in all domains of life Lambrecht, JA, Schmitz, GE, Downs, DM	MBio	2013
25975565	Genomic and experimental evidence for multiple metabolic functions in the RidA/YjgF/YER057c/UK114 (Rid) protein family Niehaus, TD, Gerdes, S, Hodge-Hanson, K, Zhukov, A, Cooper, AJ, ElBadawi-Sidhu, M, Fiehn, O, Downs, DM, Hanson, AD	BMC Genomics	2015
25620221	From microbiology to cancer biology: the Rid protein family prevents cellular damage caused by endogenously generated reactive nitrogen species Downs, DM, Ernst, DC	Mol. Microbiol.	2015
20306345	Cysteine S-conjugate β-lyases: important roles in the metabolism of naturally occurring sulfur and selenium-containing compounds, xenobiotics and anticancer agents Cooper, AJ, Krasnikov, BF, Niatsetskaya, ZV, Pinto, JT, Callery, PS, Villar, MT, Artigues, A, Bruschi, SA	Amino Acids	2011