Reactome | USP9X hydrolyzes Ub:PEX5L yielding PEX5L and Ubiquitin

USP9X hydrolyzes Ub:PEX5L yielding PEX5L and Ubiquitin

Stable Identifier

R-HSA-9033491

Type

Reaction [transition]

Species

Homo sapiens

Compartment

cytosol

ReviewStatus

5/5

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USP9X hydrolyzes Ub:PEX5L yielding PEX5L and Ubiquitin

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The deubiquitinating enzyme USP9X hydrolyzes the thioester bond between the carboxyl terminus of ubiquitin and cysteine-11 of PEX5L (Grou et al. 2012). The thioester bond is unstable and appears to be also spontaneously (non-enzymatically) disrupted by nucleophilic attack of small metabolites such as reduced glutathione (Grou et al. 2009).

Literature References

PubMed ID	Title	Journal	Year
19208625	Properties of the ubiquitin-pex5p thiol ester conjugate Huybrechts, SJ, Sá-Miranda, C, Grou, CP, Carvalho, AF, Azevedo, JE, Pinto, MP, Fransen, M	J. Biol. Chem.	2009
22371489	Identification of ubiquitin-specific protease 9X (USP9X) as a deubiquitinase acting on ubiquitin-peroxin 5 (PEX5) thioester conjugate Domingues, P, Sá-Miranda, C, Rodrigues, TA, Freitas, MO, Grou, CP, Carvalho, AF, Wood, SA, Azevedo, JE, Pinto, MP, Rodríguez-Borges, JE, Fransen, M, Francisco, T	J. Biol. Chem.	2012