Formation of meiotic holliday junction

Stable Identifier
Reaction [omitted]
Homo sapiens
Formation of Meiotic Recombination Intermediate
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The 3' end of the invading strand is extended by an unknown DNA polymerase and the extended strand is then ligated back to the original homolog, generating a double Holliday junction. MSH4 and MSH5 form heterodimers which bind Holliday junctions and, in the presence of ATP, slide along the parental duplexes (Bocker et al. 1999, Snowden et al. 2004, Snowden et al. 2008). MSH4 is present at hundreds of meiotic nodules during late zygotene but only about 10% of these nodules become crossovers (Oliver-Bonet et al. 2005). Bloom Syndrome protein (BLM) and Topoisomerase IIIa (TOP3A) are also present and may promote homologous recombination repair without crossing over (Johnson et al. 2000, Wu et al. 2000).
Literature References
PubMed ID Title Journal Year
15304223 hMSH4-hMSH5 recognizes Holliday Junctions and forms a meiosis-specific sliding clamp that embraces homologous chromosomes

Berardini, M, Butz, C, Fishel, R, Snowden, T, Acharya, S

Mol Cell 2004
17977839 hMSH4-hMSH5 adenosine nucleotide processing and interactions with homologous recombination machinery

Fishel, R, Snowden, T, Acharya, S, Schmutte, C, Shim, KS

J Biol Chem 2008
10029069 hMSH5: a human MutS homologue that forms a novel heterodimer with hMSH4 and is expressed during spermatogenesis

Fishel, R, Rasio, D, Snowden, T, Robbins, D, Copeland, T, Croce, CM, Bocker, T, Schmidt, C, Kovatich, AJ, Burczak, J, Barusevicius, A, Guerrette, S

Cancer Res 1999
10734115 The Bloom's syndrome gene product interacts with topoisomerase III

Wu, L, Turley, H, North, PS, Davies, SL, Riou, JF, Goulaouic, H, Hickson, ID, Gatter, KC

J Biol Chem 2000
16123081 Temporal progression of recombination in human males

Sun, F, Turek, PJ, Ko, E, Martin, RH, Oliver-Bonet, M

Mol Hum Reprod 2005
10728666 Association of the Bloom syndrome protein with topoisomerase IIIalpha in somatic and meiotic cells

Guarente, L, Marciniak, RA, Mastrangelo, MA, Lombard, DB, Jaenisch, R, Dewolf, W, Yin, Y, Johnson, FB, Ellis, NA, Neff, NF

Cancer Res 2000
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