Studies of the VGF promoter have identified a number of consensus sites in a minimal 110 bp promoter spanning -180 to -70 upstream of the transcriptional start site (D'Arcangelo et al, 1996; Possenti et al, 1992; Mandolesi et al, 2002). These sites, which include an E-box, a CCAAT site, a CRE element and a G(S)G site, are required for NGF-responsive transcription in neuronal cells (Possenti et al, 1992; D'Arcangelo et al, 1996; Mandolesi et al, 2002). The E box and CCAAT elements are bound by TCF12 (also known as HEB) and ASCL1 (also known as MASH1) to weakly stimulate trancriptional activity (Di Rocco et al, 1997; Mandolesi et al, 2002). The CRE is bound by phosphorylated CREB and by members of the ATF family . CRE binding is facilitated through protein-protein interactions with an unidentified CCAAT-binding factor (Di Rocco et al, 1997; D'Arcangelo et al, 1996; Mandolesi et al, 2002). The CREB:ASCL1 complex also includes the histone acetyltransferase p300 (Mandolesi et al, 2002; Adams et al, 2017).
Di Rocco, G, Pennuto, M, Illi, B, Nasi, S, Trani, E, Mandolesi, G, Sirinian, MI, Levi, A, Filocamo, G, Jucker, R, Possenti, R, Canu, N, Rinaldi, AM
Kletsov, S, Cooper, GM, Elman, JS, Adams, KW, Lamm, RJ, Mullenbrock, S
Mandolesi, G, Gargano, S, Molfetta, R, Pennuto, M, Illi, B, Nasi, S, Levi, A, Jucker, R, Mosca, L, Soucek, L
Di Rocco, G, Nasi, S, Levi, A, Possenti, R
D'Arcangelo, G, Salton, SR, Halegoua, S, Habas, R, Wang, S
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