F8 variant is not sulfonated at Y1699

Stable Identifier
Reaction [transition]
Homo sapiens
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Post-translational cellular processing of the factor VIII (FVIII or F8) precursor enables O-sulfation of tyrosine residues (Pittman DD et al. 1992; Michnick DA et al. 1994). Biochemical and structural studies demonstrated that human FVIII contains six potential tyrosine sulfation sites on the FVIII molecule, ie, four on the heavy chain (at amino acid residues 365, 737, 738, and 742) and two in the a3 subdomain of the light chain (residues 1683 and 1699) (Pittman DD et al. 1992; Michnick DA et al. 1994; Severs JC et al. 1999; Schmidbauer S et al. 2015). Site-directed mutagenesis of individual or multiple tyrosine residues showed that all the six sulfation sites are required to modulate FVIII activity (Pittman DD et al. 1992; Michnick DA et al. 1994). Further, mutagenesis of Tyr1699 to Phe (Y1699F) demonstrated that sulfation at that residue was required for high affinity interaction of FVIII with von Willebrand factor (vWF) (Leyte A et al. 1991). In the absence of tyrosine sulfation at 1699 in FVIII, the affinity for vWF was reduced by 5-fold (Leyte A et al. 1991). Nuclear magnetic resonance (NMR) spectrum studies of the complex between FVIII and vWF showed significantly larger residue-specific chemical shift changes when Y1699 was sulfated further highlighting the importance of FVIII sulfation at Y1699 for the binding affinity to vWF (Dagil L et al. 2019). The significance of the sulfation of FVIII at Y1699 in vivo is made evident by the presence of a Y1699F mutation that causes a moderate hemophilia A, likely due to reduced interaction with vWF and decreased plasma half-life (Higuchi M et al. 1990; van den Biggelaar M et al. 2011). The Reactome event describes defective O-sulfation of FVIII precursor due to mutation at Y1699.

Literature References
PubMed ID Title Journal Year
1554716 Identification and functional importance of tyrosine sulfate residues within recombinant factor VIII

Pittman, DD, Kaufman, RJ, Wang, JH

Biochemistry 1992
1898735 Sulfation of Tyr1680 of human blood coagulation factor VIII is essential for the interaction of factor VIII with von Willebrand factor

Huttner, WB, Leyte, A, van Mourik, JA, van Schijndel, HB, Niehrs, C, Verbeet, MP, Mertens, K

J. Biol. Chem. 1991
8051097 Identification of individual tyrosine sulfation sites within factor VIII required for optimal activity and efficient thrombin cleavage

Pittman, DD, Kaufman, RJ, Wise, RJ, Michnick, DA

J. Biol. Chem. 1994
Catalyst Activity

protein-tyrosine sulfotransferase activity of TPST1,2 [Golgi membrane]

Normal reaction
Functional status

Loss of function of F8 Y1699F [Golgi lumen]

Name Identifier Synonyms
factor VIII deficiency DOID:12134 Congenital factor VIII disorder, Subhemophilia, Hemophilia A
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