PELI1 ubiquitinates RIPK3 at K363

Stable Identifier
R-HSA-9686920
Type
Reaction [omitted]
Species
Homo sapiens
Compartment
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Receptor-interacting protein kinase-3 (RIP3 or RIPK3) interacts with pellino E3 ubiquitin protein ligase 1 (PELI1) through its forkhead-associated (FHA) domain to mediate K48-linked polyubiquitination of RIPK3 (Choi SW et al. 2018). Mass spectrometric analysis identified K363 of RIPK3 as the main target for PELI1-mediated ubiquitination. In vitro ubiquitination assay showed that PELI1 utilized the ubiquitin-conjugating enzyme UbcH5a to ubiquitinate RIPK3. Proteasome inhibitors (MG132 and BTZ), but not lysosome inhibitors, prevented PELI1-facilitated degradation of stably expressed RIPK3 in HeLa cells and endogenous RIPK3 in HT-29 cells, suggesting that PELI1 controls RIP3 protein destabilization via ubiquitylation-dependent proteasome-mediated degradation (Choi SW et al. 2018). Phosphorylation of RIPK3 at T182 was necessary for PELI1 recruitment and K48-linked polyubiquitination. PELI1-mediated RIPK3 degradation abolished the phosphorylation of mixed lineage kinase domain like pseudokinase (MLKL) and necroptotic cell death in human colorectal adenocarcinoma (HT29) cells, human neonatal epidermal keratinocytes (HEKn), and in lung tissues from PELI1 transgenic mice (Choi SW et al. 2018).

Literature References
PubMed ID Title Journal Year
29883609 PELI1 Selectively Targets Kinase-Active RIP3 for Ubiquitylation-Dependent Proteasomal Degradation

Choi, SW, Park, HH, Kim, S, Chung, JM, Noh, HJ, Kim, SK, Song, HK, Lee, CW, Morgan, MJ, Kang, HC, Kim, YS

Mol. Cell 2018
Participants
Participates
Catalyst Activity

ubiquitin protein ligase activity of p-S-RIPK1:p-S199,227-RIPK3:PELI1 [cytosol]

Orthologous Events
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