Spliceosomal P complex dissociates yielding the intron-containing complex (ILS) and the spliced mRNP (new)

Stable Identifier
R-HSA-9770847
Type
Reaction [uncertain]
Species
Homo sapiens
Compartment
ReviewStatus
5/5
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DHX8 (homolog of yeast Prp22) hydrolyzes ATP and translocates along the RNA in a 3' to 5' direction (Felisberto-Rodrigues et al. 2019) to release the spliced mRNA and associated proteins (Merz et al. 2007), including the exon junction complex (EJC), from the Spliceosomal P complex (Ohno and Shimura 1996, Zhang et al. 2019). After the second splicing reaction, DHX8 interacts with CACTIN and SRRM2 in the Spliceosomal P complex and is bound to the 3' exon downstream of the 3' splice site. As DHX8 translocates along the RNA, it is thought to pull the 3' end of the spliced exon and dissociate the spliced exon from the U5 snRNA. SLU7 and PRPF18 are also dissociated from the spliceosome at this time. The intron lariat remains bound to the intron lariat spliceosome (ILS) (Yoshimoto et al. 2009). TFIP11 (homolog of yeast Ntr1) is present in the ILS (Yoshimoto et al. 2009) and recruits DDX15 (homolog of yeast Prp43) to disassemble the ILS.
Literature References
PubMed ID Title Journal Year
30728453 Structures of the human spliceosomes before and after release of the ligated exon

Shi, Y, Liu, D, Yan, C, Zhang, W, Zhang, X, Lei, J, Zhan, X

Cell Res 2019
17095540 Protein composition of human mRNPs spliced in vitro and differential requirements for mRNP protein recruitment

Will, CL, L├╝hrmann, R, Urlaub, H, Merz, C

RNA 2007
8608946 A human RNA helicase-like protein, HRH1, facilitates nuclear export of spliced mRNA by releasing the RNA from the spliceosome

Ohno, M, Shimura, Y

Genes Dev 1996
19103666 Isolation and characterization of post-splicing lariat-intron complexes

Ohno, M, Yoshimoto, R, Okawa, K, Kataoka, N

Nucleic Acids Res. 2009
28062851 Human cactin interacts with DHX8 and SRRM2 to assure efficient pre-mRNA splicing and sister chromatid cohesion

Zanini, IM, Lorenzi, LE, Azzalin, CM, Soneson, C

J Cell Sci 2017
31409651 Structural and functional characterisation of human RNA helicase DHX8 provides insights into the mechanism of RNA-stimulated ADP release

Thomas, JC, Workman, P, Felisberto-Rodrigues, C, Le Bihan, YV, Burke, R, McAndrew, C, van Montfort, RLM

Biochem J 2019
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Catalyst Activity

ATP-dependent activity, acting on RNA of Spliceosomal P complex [nucleoplasm]

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