Reactome | Spliceosomal P complex dissociates yielding the intron-containing complex (ILS) and the spliced mRNP (new)

Spliceosomal P complex dissociates yielding the intron-containing complex (ILS) and the spliced mRNP (new)

Stable Identifier

R-HSA-9770847

Type

Reaction [uncertain]

Species

Homo sapiens

Compartment

nucleoplasm

ReviewStatus

5/5

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Spliceosomal P complex dissociates yielding the intron-containing complex (ILS) and the spliced mRNP (new)

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DHX8 (homolog of yeast Prp22) hydrolyzes ATP and translocates along the RNA in a 3' to 5' direction (Felisberto-Rodrigues et al. 2019) to release the spliced mRNA and associated proteins (Merz et al. 2007), including the exon junction complex (EJC), from the Spliceosomal P complex (Ohno and Shimura 1996, Zhang et al. 2019). After the second splicing reaction, DHX8 interacts with CACTIN and SRRM2 in the Spliceosomal P complex and is bound to the 3' exon downstream of the 3' splice site. As DHX8 translocates along the RNA, it is thought to pull the 3' end of the spliced exon and dissociate the spliced exon from the U5 snRNA. SLU7 and PRPF18 are also dissociated from the spliceosome at this time. The intron lariat remains bound to the intron lariat spliceosome (ILS) (Yoshimoto et al. 2009). TFIP11 (homolog of yeast Ntr1) is present in the ILS (Yoshimoto et al. 2009) and recruits DDX15 (homolog of yeast Prp43) to disassemble the ILS.

Literature References

PubMed ID	Title	Journal	Year
30728453	Structures of the human spliceosomes before and after release of the ligated exon Zhang, X, Zhan, X, Yan, C, Zhang, W, Liu, D, Lei, J, Shi, Y	Cell Res	2019
17095540	Protein composition of human mRNPs spliced in vitro and differential requirements for mRNP protein recruitment Merz, C, Urlaub, H, Will, CL, Lührmann, R	RNA	2007
8608946	A human RNA helicase-like protein, HRH1, facilitates nuclear export of spliced mRNA by releasing the RNA from the spliceosome Ohno, M, Shimura, Y	Genes Dev	1996
19103666	Isolation and characterization of post-splicing lariat-intron complexes Yoshimoto, R, Kataoka, N, Okawa, K, Ohno, M	Nucleic Acids Res.	2009
28062851	Human cactin interacts with DHX8 and SRRM2 to assure efficient pre-mRNA splicing and sister chromatid cohesion Zanini, IM, Soneson, C, Lorenzi, LE, Azzalin, CM	J Cell Sci	2017
31409651	Structural and functional characterisation of human RNA helicase DHX8 provides insights into the mechanism of RNA-stimulated ADP release Felisberto-Rodrigues, C, Thomas, JC, McAndrew, C, Le Bihan, YV, Burke, R, Workman, P, van Montfort, RLM	Biochem J	2019