Disassembly of the Intron Lariat Spliceosome (new)

Stable Identifier
R-HSA-9772351
Type
Reaction [transition]
Species
Homo sapiens
Compartment
nucleoplasm
ReviewStatus
5/5
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Disassembly of the Intron Lariat Spliceosome (new)
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DHX15 (the homolog of yeast Prp43) hydrolyzes ATP (Studer et al. 2020) and translocates along U6 snRNA (inferred from yeast Prp43 in Toroney et al. 2019) to release the lariat intron from the Intron Lariat Spliceosome (ILS) (Yoshimoto et al. 2009). TFIP11 (homolog of yeast Ntr1) and GCFC2 (C2ORF3, homolog of yeast Ntr2) bind and stimulate DHX15 activity (Wen et al. 2008, Yoshimoto et al. 2014). The disassembly process is observed as a shift from the ILS to the Intron Small complex., which contains the lariat intron and some undefined proteins (Yoshimoto et al. 2009). The PRP19-CDC5L complex (NTC), the PRP19-CDC5L related complex (NTR), the U5 snRNP, U2 12S snRNP, and U6 snRNA are released and reused in subsequent splicing reactions (Yoshimoto et al. 2009).The intron lariat is debranched (linearized) by the 2'-5' phosphodiesterase DBR1 (Kataoka et al. 2013) and then degraded by specific decay machineries.
Literature References
PubMed ID Title Journal Year
19165350 TFIP11 interacts with mDEAH9, an RNA helicase involved in spliceosome disassembly

Wen, X, Tannukit, S, Paine, ML

Int J Mol Sci 2008
32179686 Structural basis for DEAH-helicase activation by G-patch proteins

Studer, MK, Ivanović, L, Weber, ME, Marti, S, Jonas, S

Proc Natl Acad Sci U S A 2020
19103666 Isolation and characterization of post-splicing lariat-intron complexes

Yoshimoto, R, Kataoka, N, Okawa, K, Ohno, M

Nucleic Acids Res. 2009
30728453 Structures of the human spliceosomes before and after release of the ligated exon

Zhang, X, Zhan, X, Yan, C, Zhang, W, Liu, D, Lei, J, Shi, Y

Cell Res 2019
24304693 Identification of a novel component C2ORF3 in the lariat-intron complex: lack of C2ORF3 interferes with pre-mRNA splicing via intron turnover pathway

Yoshimoto, R, Okawa, K, Yoshida, M, Ohno, M, Kataoka, N

Genes Cells 2014
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Catalyst Activity

ATP-dependent activity, acting on RNA of Intron Lariat Spliceosome (ILS) [nucleoplasm]

Physical Entity
Intron Lariat Spliceosome (ILS) [nucleoplasm] (Homo sapiens)
Activity
ATP-dependent activity, acting on RNA (GO:0008186)
Authored
May, B  (2022-04-21)
Reviewed
Charenton, C  (2022-10-20)
Created
May, B  (2022-04-21)
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