Expression of homeobox protein NOTO is activated by FOXA2 and TBXT (T, BRACHYURY) (inferred from mouse homologs). NOTO is activated during in vitro differentiation of human notochord cells (Diaz-Hernandez et al. 2020). TEAD2:YAP and LEF1:CTNNB1 or TCF7:CTNNB1 activate the NOTO promoter in vitro but appear to be dispensable for activation in the node and notochord in vivo (inferred from mouse homologs). NOTO represses paraxial mesoderm fate and maintains axial mesoderm fate (inferred from mouse embryos). Knockout of Noto in mice demonstrates that Noto regulates node morphogenesis, notochordal ciliagenesis, and left-right patterning (Beckers et al. 2007). Part of this regulation is accomplished by the activation of cilia-related genes in the node and posterior region of the notochord by Noto (Beckers et al. 2007).