Formation of the NSL complex

Stable Identifier
Reaction [transition]
Homo sapiens
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WDR5 is a subunit of an evolutionarily conserved lysine acetyltransferase Non-Specific-Lethal (NSL) complex together with the following proteins: KAT8 (also known as MOF) as the catalytic subunit, KANSL1-3 (also known as NSL1-3), MCRS1, HCFC1 (also known as Host cell factor 1 or HCF1), and one of the paralogous proteins: PHF20 or PHF20-like 1 (PHF20L1) (Smith et al, 2005; Cai et al, 2010; Van et al 2022). The NSL complex is responsible for the acetylation of lysine K5, K8, K12, and K16 of histone H4 (Smith et al, 2005; Cai et al, 2010; Zhao et al, 2013; Radzisheuskaya et al., 2021). The NSL complex binds to the promoters of house-keeping genes and regulates their expression (Radzisheuskaya et al., 2021), therefore in some contexts, it is essential for the viability and proliferation of human cells (Klein et al, 2016; Radzisheuskaya et al., 2021).
Literature References
PubMed ID Title Journal Year
35033534 Methyl-lysine readers PHF20 and PHF20L1 define two distinct gene expression-regulating NSL complexes

Lu, Y, Jain, AK, Van, HT, Bedford, MT, Santos, MA, Patel, A, Harkins, PR

J Biol Chem 2022
20018852 Subunit composition and substrate specificity of a MOF-containing histone acetyltransferase distinct from the male-specific lethal (MSL) complex

Choi, SH, Cai, Y, Conaway, RC, Florens, LA, Conaway, JW, Swanson, SK, Cole, MD, Washburn, MP, Jin, J

J. Biol. Chem. 2010
27760318 PHF20 Readers Link Methylation of Histone H3K4 and p53 with H4K16 Acetylation

Wang, X, Cui, G, Mer, G, Lu, Y, Kutateladze, TG, Klein, BJ, Shi, X, Yuan, C, Wang, X, Bruns, CJ, Zhao, Y, Lin, K, Botuyan, MV

Cell Rep 2016
16227571 A human protein complex homologous to the Drosophila MSL complex is responsible for the majority of histone H4 acetylation at lysine 16

Lane, WS, Smith, ER, Côté, J, Huang, R, Cayrou, C, Lucchesi, JC

Mol. Cell. Biol. 2005
33657400 Complex-dependent histone acetyltransferase activity of KAT8 determines its role in transcription and cellular homeostasis

Helin, K, Hendrickson, RC, Gorshkov, V, Jensen, ON, Johnstone, AL, Kovalchuk, S, Grinev, VV, Zhan, Y, Rodriguez, KL, Shliaha, PV, Radzisheuskaya, A, Shlyueva, D, Koche, R, Keogh, MC, Damhofer, H

Mol Cell 2021
24244196 Crosstalk between NSL histone acetyltransferase and MLL/SET complexes: NSL complex functions in promoting histone H3K4 di-methylation activity by MLL/SET complexes

Cai, Y, Wu, M, Conaway, RC, Yang, Y, Jin, J, Cao, L, Liu, D, Ding, J, Conaway, JW, Wu, D, Wang, F, Zhao, X, Su, J

PLoS Genet 2013
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