Transfer of Ub from E2 to substrate and release of E2

Stable Identifier
R-HSA-983140
Type
Reaction [transition]
Species
Homo sapiens
Compartment
cytosol
ReviewStatus
5/5
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Transfer of Ub from E2 to substrate and release of E2
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Interaction of E3 with both substrate and E2-Ub, brings them into proximity so that ubiquitin is transferred from E2 to the substrate. In most cases the transfer of ubiquitin is direct from E2 to substrate, but in a small subset of E3s, it occurs via a covalent E3-Ub thioester intermediate (Raymond et al. 2009).

Literature References
PubMed ID Title Journal Year
12646216 U-box proteins as a new family of ubiquitin ligases

Hatakeyama, S, Nakayama, KI

Biochem Biophys Res Commun 2003
16954532 A novel family of membrane-bound E3 ubiquitin ligases

Goto, E, Hotta, H, Ishido, S, Mito, M, Uematsu, M, Aoki, M, Ohmura-Hoshino, M, Matsuki, Y

J Biochem 2006
19489725 RING domain E3 ubiquitin ligases

Deshaies, RJ, Joazeiro, CA

Annu Rev Biochem 2009
Participants
Input
Output
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as an event of
Catalyst Activity

ubiquitin-protein transferase activity of E3 ligases in proteasomal degradation [cytosol]

Physical Entity
E3 ligases in proteasomal degradation [cytosol] (Homo sapiens)
Activity
ubiquitin-protein transferase activity (GO:0004842)
Orthologous Events
Transfer of Ub from E2 to substrate and release of E2 (Bos taurus)
Transfer of Ub from E2 to substrate and release of E2 (Caenorhabditis elegans)
Transfer of Ub from E2 to substrate and release of E2 (Canis familiaris)
Transfer of Ub from E2 to substrate and release of E2 (Danio rerio)
Transfer of Ub from E2 to substrate and release of E2 (Dictyostelium discoideum)
Transfer of Ub from E2 to substrate and release of E2 (Drosophila melanogaster)
Transfer of Ub from E2 to substrate and release of E2 (Gallus gallus)
Transfer of Ub from E2 to substrate and release of E2 (Mus musculus)
Transfer of Ub from E2 to substrate and release of E2 (Plasmodium falciparum)
Transfer of Ub from E2 to substrate and release of E2 (Rattus norvegicus)
Transfer of Ub from E2 to substrate and release of E2 (Sus scrofa)
Transfer of Ub from E2 to substrate and release of E2 (Xenopus tropicalis)
Authored
Garapati, P V  (2010-10-29)
Reviewed
Elliott, T  (2011-02-11)
Created
Garapati, P V  (2010-10-29)
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