Formation of the 26S proteasome

Stable Identifier
R-HSA-9908178
Type
Reaction [omitted]
Species
Homo sapiens
Compartment
cytosol
ReviewStatus
3/5
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Formation of the 26S proteasome
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In the final step of the 26S proteasome assembly, 19S regulatory particle precursor (19S RP) binds to the 20S core particle (20S CP). Through a poorly elucidated mechanism, chaperone proteins PAAF1 (ortholog of yeast Rpn14), PSMD10 (ortholog of yeast Nas6), and PSMD5 (ortholog of yeast Hsm3) are released from the base precursor and are not found in the 26S proteasome (reviewed in Tomko and Hochstrasser 2013). The cryogenic electron microscopy (cryo-EM) analysis of the mature human 19S RP confirmed that PAAF1 (Rpn14), PSMD10 (Nas6), and PSMD5 (Hsm3) were absent (Lu et al. 2017), implying that they are only transiently associated with 19S RP subunits, as shown in yeast.
Literature References
PubMed ID Title Journal Year
28689658 Conformational Landscape of the p28-Bound Human Proteasome Regulatory Particle

Lu, Y, Wu, J, Dong, Y, Chen, S, Sun, S, Ma, YB, Ouyang, Q, Finley, D, Kirschner, MW, Mao, Y

Mol Cell 2017
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Orthologous Events
Formation of the 26S proteasome (Dictyostelium discoideum)
Authored
Orlic-Milacic, M  (2024-04-22)
Created
Orlic-Milacic, M  (2024-04-19)
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