Reactome | DAG1(30-653), DAG1(654-895) and SSPN bind SGC

DAG1(30-653), DAG1(654-895) and SSPN bind SGC

Stable Identifier

R-HSA-9913332

Type

Reaction [binding]

Species

Homo sapiens

Compartment

Golgi lumen, Golgi membrane

ReviewStatus

5/5

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DAG1(30-653), DAG1(654-895) and SSPN bind SGC

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In the Golgi, the SGC interacts with alpha- and beta-dystroglycan (DAG1(30-653) and DAG1(654-895)) (Noguchi et al, 2000; reviewed in Tarakci and Bergman, 2016). DAG1 is autocatalytically proteolyzed during transit through the ER, yielding the alpha and beta fragments, but these remain associated through a non-covalent interaction of the C-terminal region of the alpha subunit with the N-terminal region of the beta subunit (Holt et al, 2000; Sciandra et al, 2001; Herzog et al, 2004; Akhavan et al, 2008). Alpha-DAG1 is additionally heavily glycosylated during transit through the secretory system (reviewed in Praissman and Wells, 2014).
In addition to alpha- and beta-dystroglycan, SSPN (sarcospan) is also recruited to the assembling complex in the Golgi. SSPN has four transmembrane segments and a large extracellular loop and is believed to mediate the interaction between the SGC and DAG1 complex (Crosbie et al, 1999; Crosbie et al, 2000).

Literature References

PubMed ID	Title	Journal	Year
15175026	Dystroglycan in skin and cutaneous cells: beta-subunit is shed from the cell surface Kröger, S, Franzke, CW, Bruckner-Tuderman, L, Echtermeyer, FG, Gustafsson, E, Fässler, R, Herzog, C, Schlötzer-Schrehardt, U, Has, C	J Invest Dermatol	2004
10189375	Membrane targeting and stabilization of sarcospan is mediated by the sarcoglycan subcomplex Crosbie, RH, Campbell, KP, Holt, KH, Lebakken, CS, Grady, RM, Lee, JC, Straub, V, Venzke, DP, Sanes, JR, Chamberlain, JS	J Cell Biol	1999
24786756	Mammalian O-mannosylation pathway: glycan structures, enzymes, and protein substrates Wells, L, Praissman, JL	Biochemistry	2014
10942431	Molecular and genetic characterization of sarcospan: insights into sarcoglycan-sarcospan interactions Crosbie, RH, Campbell, KP, Hirano, M, Lim, LE, Collin, H, Stolle, CA, Moore, SA, Hays, AP, Maybaum, SW, Fardeau, M, Dovico, SA, Tomé, FM	Hum Mol Genet	2000
11502221	Identification of the beta-dystroglycan binding epitope within the C-terminal region of alpha-dystroglycan Brancaccio, A, Sciandra, F, Baumgartner, S, Petrucci, TC, Schneider, M, Giardina, B	Eur J Biochem	2001
26709803	The sarcoglycan complex in skeletal muscle Tarakci, H, Berger, J	Front Biosci (Landmark Ed)	2016
10651799	Formation of sarcoglycan complex with differentiation in cultured myocytes Imamura, M, Noguchi, S, Ozawa, E, Wakabayashi, E, Yoshida, M	Eur J Biochem	2000
17905726	SEA domain proteolysis determines the functional composition of dystroglycan Crivelli, SN, Muschler, JL, Akhavan, A, Singh, M, Lingappa, VR	FASEB J	2008