Reactome | DGC complex binds laminins

DGC complex binds laminins

Stable Identifier

R-HSA-9913333

Type

Reaction [binding]

Species

Homo sapiens

Compartment

plasma membrane, extracellular region

ReviewStatus

5/5

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DAG1 is present in the dystroglycan-associated glycoprotein complex (DGC) as non-covalently associated alpha and beta subunits following cleavage at Ser654. The extracellular alpha subunit binds to LAMA2 (laminin-2, also known as merosin) in the muscle basement membrane while the membrane-associated beta subunit binds dystrophin (DMD), which associates with the actin cytoskeleton (Ervasti & Campbell 1993, Yamada et al. 1994, Talts et al. 1999). Alpha-DAG1 also binds the carboxy-terminal G domains of laminin alpha-1 (LAMA1; Gee et al. 1993, Zhou et al. 2012) and alpha-5 (LAMA5; Yu & Talts 2003). G domains are relatively well conserved in all five alpha-laminin chains, so alpha-DAG1 is likely to bind all laminin heterotrimers. Gycosylation of the alpha-DAG1 subunit is critical for interactions with laminins (Yoshida-Moriguchi et al, 2010; Inamori et al, 2012).

Literature References

PubMed ID	Title	Journal	Year
8349731	A role for the dystrophin-glycoprotein complex as a transmembrane linker between laminin and actin Ervasti, JM, Campbell, KP	J. Cell Biol.	1993
22159078	Laminin-?1 LG4-5 domain binding to dystroglycan mediates muscle cell survival, growth, and the AP-1 and NF-?B transcription factors but also has adverse effects Jarrett, HW, Zhou, YW, Munoz, J, Jiang, D	Am. J. Physiol., Cell Physiol.	2012
8325873	Laminin-binding protein 120 from brain is closely related to the dystrophin-associated glycoprotein, dystroglycan, and binds with high affinity to the major heparin binding domain of laminin Provost, PR, Douville, PJ, Gee, SH, Blacher, RW, Carbonetto, S, Yurchenco, PD	J. Biol. Chem.	1993
22223806	Dystroglycan function requires xylosyl- and glucuronyltransferase activities of LARGE Inamori, K, Yoshida-Moriguchi, T, Anderson, ME, Yu, L, Hara, Y, Campbell, KP	Science	2012
10022829	Binding of the G domains of laminin alpha1 and alpha2 chains and perlecan to heparin, sulfatides, alpha-dystroglycan and several extracellular matrix proteins Brancaccio, A, Timpl, R, Talts, JF, Göhring, W, Andac, Z	EMBO J.	1999
7925941	Dystroglycan is a binding protein of laminin and merosin in peripheral nerve Yamada, H, Matsumura, K, Shimizu, T, Tanaka, T, Campbell, KP	FEBS Lett.	1994
20044576	O-mannosyl phosphorylation of alpha-dystroglycan is required for laminin binding Stalnaker, SH, Yoshida-Moriguchi, T, Madson, M, Wells, L, Kunz, S, Oldstone, MB, Yu, L, Schachter, H, Campbell, KP, Davis, S	Science	2010
12519075	Beta1 integrin and alpha-dystroglycan binding sites are localized to different laminin-G-domain-like (LG) modules within the laminin alpha5 chain G domain Talts, JF, Yu, H	Biochem. J.	2003