Expression of Pparg

Stable Identifier
R-MMU-442475
Type
Reaction [omitted]
Species
Mus musculus
Compartment
nucleoplasm
ReviewStatus
5/5
General
SVG | 
PNG
Low
Medium
High
 | PPTX  | SBGN
Expression of Pparg
In mouse 3T3-L1 cells, the transcription factors Cebpb, Cebpd, and Klf5 simultaneously bind the Pparg promoter and synergistically activate transcription of the Pparg gene (Oishi et al. 2005, Wu et al.1996, Hamm et al. 2001, Wu et al. 1995).These three factors activate transcription after initial stimulation of adipocyte differentiation but then are replaced by Cebpa within 10 days (Yeh et al. 1995). Cebpa and other factors may be responsible for long term maintenance of Pparg expression and the differentiated state.
Preadipose tissue contains both the widely expressed Pparg variant 1 mRNA and the more tissue-specific Pparg variant 2. The Pparg variant 2 mRNA is translated to yield Pparg variant 2 protein, which has 505 amino acid residues (57 KDa) and is the longest of the 4 observed variants. Variant 2 is specific to preadipose tissue.
In mouse 3T3-L1 cells, by 10 days after induction of adipocyte differentiation Cebpa, but neither Cebpb nor Cebpd, is detectable at the Pparg promoter (Yeh et al. 1995). While adipocyte differentiation can proceed without Cebpa, adipocytes differentiated from Cebpa-knockout cells are insulin insensitive due to a defect in Glut4 (Slc2a4) vesicle trafficking (Wertheim et al. 2004).
Znf638 cooperates together with Cebpb and Cebpd at the promoter of the Pparg gene to activate transcription (Meruvu et al. 2011).
Literature References
PubMed ID Title Journal Year
8754811 Induction of peroxisome proliferator-activated receptor gamma during the conversion of 3T3 fibroblasts into adipocytes is mediated by C/EBPbeta, C/EBPdelta, and glucocorticoids

Wu, Z, Bucher, NL, Farmer, SR

Mol Cell Biol 1996
15277525 The transcription factor CCAAT/enhancer-binding protein alpha is required for the intracellular retention of GLUT4

Wertheim, N, Cai, Z, McGraw, TE

J Biol Chem 2004
16054042 Krüppel-like transcription factor KLF5 is a key regulator of adipocyte differentiation

Oishi, Y, Manabe, I, Tobe, K, Tsushima, K, Shindo, T, Fujiu, K, Nishimura, G, Maemura, K, Yamauchi, T, Kubota, N, Suzuki, R, Kitamura, T, Akira, S, Kadowaki, T, Nagai, R

Cell Metab 2005
7531665 Cascade regulation of terminal adipocyte differentiation by three members of the C/EBP family of leucine zipper proteins

Yeh, WC, Cao, Z, Classon, M, McKnight, SL

Genes Dev 1995
7557387 Conditional ectopic expression of C/EBP beta in NIH-3T3 cells induces PPAR gamma and stimulates adipogenesis

Wu, Z, Xie, Y, Bucher, NL, Farmer, SR

Genes Dev 1995
11279134 A role for C/EBPbeta in regulating peroxisome proliferator-activated receptor gamma activity during adipogenesis in 3T3-L1 preadipocytes

Hamm, JK, Park, BH, Farmer, SR

J Biol Chem 2001
17011499 Transcriptional control of adipocyte formation

Farmer, SR

Cell Metab 2006
Participants
Input
Output
This event is regulated
Negatively by
Regulator
Wnt-1 or Wnt-10b [extracellular region] (Mus musculus)
Regulator
Tnf [extracellular region] (Mus musculus)
Regulator
Tgfb1 [extracellular region] (Mus musculus)
Positively by
Regulator
Klf5 [nucleoplasm] (Mus musculus)
Regulator
Cebpb [nucleoplasm] (Mus musculus)
Regulator
Cebpa [nucleoplasm] (Mus musculus)
Regulator
Znf638 [nucleoplasm] (Mus musculus)
Regulator
Ebf1 [nucleoplasm] (Mus musculus)
Regulator
Cebpd [nucleoplasm] (Mus musculus)
Regulator
Znf467 [nucleoplasm] (Mus musculus)
Orthologous Events
Expression of PPARG (Homo sapiens)
Authored
May, B  (2009-05-15)
Reviewed
Sethi, JK  (2011-02-10)
Created
May, B  (2009-09-27)
Cite Us!