Tet1,2,3 oxidizes 5-formylcytosine to 5-carboxylcytosine

Stable Identifier
R-MMU-5220936
Type
Reaction [transition]
Species
Mus musculus
Compartment
nucleoplasm
ReviewStatus
5/5
General
SVG | 
PNG
Low
Medium
High
 | PPTX  | SBGN
Tet1,2,3 oxidizes 5-formylcytosine to 5-carboxylcytosine
Tet1, Tet2, and Tet3 each oxidize 5-formylcytosine (5-fC) in DNA using molecular oxygen and 2-oxoglutarate to yield 5-carboxylcytosine (5-caC), carbon dioxide, and succinate (He et al. 2011, Ito et al. 2011, Crawford et al. 2016). 5-caC is observed at major satellite repeats (Shen et al. 2013). 5-caC is generated in zygotes and its persistence indicates it may be passively removed by dilution during DNA replication (Inoue et al. 2011).
Literature References
PubMed ID Title Journal Year
21817016 Tet-mediated formation of 5-carboxylcytosine and its excision by TDG in mammalian DNA

Li, BZ, Tang, Q, Sun, Y, Jia, Y, Li, X, Chen, Z, Song, CX, Wang, Y, Li, Z, Liu, P, Zhang, K, Ding, J, Li, L, Dai, Q, Xu, GL, He, C, He, YF

Science 2011
22124233 Generation and replication-dependent dilution of 5fC and 5caC during mouse preimplantation development

Shen, L, Dai, Q, Zhang, Y, He, C, Inoue, A

Cell Res. 2011
23602152 Genome-wide analysis reveals TET- and TDG-dependent 5-methylcytosine oxidation dynamics

Diep, D, Fung, HL, Wu, H, Shen, L, Zhang, K, Yamaguchi, S, D'Alessio, AC, Zhang, Y

Cell 2013
26734843 Tet2 Catalyzes Stepwise 5-Methylcytosine Oxidation by an Iterative and de novo Mechanism

Garcia, BA, Crawford, DJ, Kohli, RM, Liu, MY, Cao, XJ, Nabel, CS

J. Am. Chem. Soc. 2016
21778364 Tet proteins can convert 5-methylcytosine to 5-formylcytosine and 5-carboxylcytosine

Shen, L, Ito, S, Dai, Q, Zhang, Y, He, C, Wu, SC, Swenberg, JA, Collins, LB

Science 2011
Participants
Input
Output
Event Information
Go Biological Process
DNA demethylation (0080111)
Catalyst Activity

methylcytosine dioxygenase activity of Tet1,2,3 [nucleoplasm]

Physical Entity
Tet1,2,3 [nucleoplasm] (Mus musculus)
Activity
methylcytosine dioxygenase activity (GO:0070579)
Orthologous Events
TET1,2,3 oxidizes 5-formylcytosine to 5-carboxylcytosine (Homo sapiens)
Authored
May, B  (2013-12-29)
Reviewed
Mukherji, M  (2014-02-21)
Pfeifer, GP  (2014-01-29)
Created
May, B  (2013-12-30)
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