Formation of Double-strand Break in DNA by Spo11

Stable Identifier
Reaction [transition]
Mus musculus
SVG |   | PPTX  | SBGN
Formation of Double-strand Break in DNA by Spo11
The gene encoding Spo11 shares sequence similarity to TopoVI, a type II topoisomerase. A Spo11 dimer cleaves both strands of DNA. Each subunit of the dimer remains covalently attached to the 5' end of one strand of DNA via a phosphodiester linkage to a conserved tyrosine residue of Spo11 (Romanienko and Camerini-Otero 2000, Baudat et al. 2000, Mahadevaiah et al. 2001, Zenvirth et al. 2003). In addition to Spo11, work from budding yeast has shown a total of 7 proteins essential for double strand break formation. The mammalian ortholog of Mei4 (S. cerevisiae) as well as a mammalian specific gene called Mei1 are essential to formation of meiotic double strand breaks (Kumar et al.2010, Libby et al. 2003).
Literature References
PubMed ID Title Journal Year
14668445 Positional cloning and characterization of Mei1, a vertebrate-specific gene required for normal meiotic chromosome synapsis in mice

Libby, BJ, Schimenti, JC, Reinholdt, LG

Proc Natl Acad Sci U S A 2003
11242108 Recombinational DNA double-strand breaks in mice precede synapsis

Baudat, F, Bonner, WM, de Boer, P, Turner, JM, Burgoyne, PS, Mahadevaiah, SK, Keeney, S, Blanco-Rodriguez, J, Jasin, M, Rogakou, EP

Nat Genet 2001
11106738 The mouse Spo11 gene is required for meiotic chromosome synapsis

Camerini-Otero, RD, Romanienko, PJ

Mol Cell 2000
12644952 Mammalian meiosis involves DNA double-strand breaks with 3' overhangs

Simchen, G, Baudat, F, Richler, C, Bardhan, A, Wahrman, J, Zenvirth, D, Barzilai, A

Chromosoma 2003
20551173 Functional conservation of Mei4 for meiotic DNA double-strand break formation from yeasts to mice

de Massy, B, Kumar, R, Bourbon, HM

Genes Dev 2010
11106739 Chromosome synapsis defects and sexually dimorphic meiotic progression in mice lacking Spo11

Baudat, F, Yuen, JP, Manova, K, Keeney, S, Jasin, M

Mol Cell 2000
Catalyst Activity

endodeoxyribonuclease activity, producing 5'-phosphomonoesters of Spo11 Dimer [nucleoplasm]

Orthologous Events
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