Removal of SPO11 and Resection of 5' Ends of DNA (yeast)

Stable Identifier
Reaction [omitted]
Saccharomyces cerevisiae
SVG |   | PPTX  | SBGN
Removal of SPO11 and Resection of 5' Ends of DNA (yeast)
SPO11 forms a dimer and each subunit cleaves a single strand of DNA, thus creating a double-strand break. After cleaving DNA, a SPO11 subunit remains covalently attached to each 5' end via a tyrosine residue. SPO11 is removed from the DNA by cleavage of single strands 3' to the attached SPO11. The products are a resected 5' end (protruding 3' overhang) and a covalent complex of SPO11 with an oligonucleotide.
Literature References
PubMed ID Title Journal Year
16107854 Endonucleolytic processing of covalent protein-linked DNA double-strand breaks

Pan, J, Neale, MJ, Keeney, S

Nature 2005
20150422 Processing of meiotic DNA double strand breaks requires cyclin-dependent kinase and multiple nucleases

Guerini, I, Citterio, A, Manfrini, N, Longhese, MP, Lucchini, G

J Biol Chem 2010
15852023 The Rad50 hook domain is a critical determinant of Mre11 complex functions

Hohl, M, Petrini, JH, Fleming, JC, Wiltzius, JJW

Nat Struct Mol Biol 2005
9858579 The nuclease activity of Mre11 is required for meiosis but not for mating type switching, end joining, or telomere maintenance

Moreau, S, Symington, LS, Ferguson, JR

Mol Cell Biol 1999
18245357 Functional interactions between Sae2 and the Mre11 complex

Weil, C, Kim, HS, Harrison, JC, Reger, M, Petrini, JH, Vijayakumar, S, Haber, JE

Genetics 2008
18670132 Sae2p phosphorylation is crucial for cooperation with Mre11p for resection of DNA double-strand break ends during meiotic recombination in Saccharomyces cerevisiae

Terasawa, M, Ogawa, H, Ogawa, T, Tsukamoto, Y

Genes Genet Syst 2008
Catalyst Activity

DNA endonuclease activity of MRX:SAE2 Complex [nucleoplasm]

3'-5'-DNA exonuclease activity of MRX:SAE2 Complex [nucleoplasm]

Orthologous Events
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