Reactome | UniProt:P60520 GABARAPL2

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UniProt:P60520 GABARAPL2 Show undefined attributes

chain	chain:1-116 propeptide:-
checksum	17ACB540FD5B975B
comment	FUNCTION Ubiquitin-like modifier involved in intra-Golgi traffic (By similarity). Modulates intra-Golgi transport through coupling between NSF activity and SNAREs activation (By similarity). It first stimulates the ATPase activity of NSF which in turn stimulates the association with GOSR1 (By similarity). Involved in autophagy (PubMed:20418806, PubMed:23209295). Plays a role in mitophagy which contributes to regulate mitochondrial quantity and quality by eliminating the mitochondria to a basal level to fulfill cellular energy requirements and preventing excess ROS production (PubMed:20418806, PubMed:23209295). Whereas LC3s are involved in elongation of the phagophore membrane, the GABARAP/GATE-16 subfamily is essential for a later stage in autophagosome maturation (PubMed:20418806, PubMed:23209295).SUBUNIT Monomer. Interacts with ATG3, ATG7, ATG13 and ULK1 (PubMed:11096062, PubMed:11146101, PubMed:11825910, PubMed:12507496, PubMed:23043107). Interacts with TP53INP1 and TP53INP2 (PubMed:19056683, PubMed:22421968, PubMed:22470510). Interacts with TBC1D25 (PubMed:21383079). Directly interacts with SQSTM1 and BNIP3 (PubMed:17580304, PubMed:23209295). Interacts with TECPR2 and PCM1 (PubMed:20562859, PubMed:24089205). Interacts with TBC1D5 (PubMed:22354992). Interacts with TRIM5 (PubMed:25127057). Interacts with MEFV and TRIM21 (PubMed:26347139). Interacts with WDFY3 (PubMed:24668264). Interacts with UBA5; promoting recruitment of UBA5 to the endoplasmic reticulum membrane (PubMed:26929408, PubMed:30990354). Interacts with GOSR1 (By similarity). Interacts with KBTBD6 and KBTBD7; the interaction is direct (PubMed:25684205). Interacts with reticulophagy regulators RETREG1, RETREG2 and RETREG3 (PubMed:34338405). Interacts with IRGM (PubMed:29420192). Interacts with DNM2 (PubMed:32315611). Interacts with NCOA4 (By similarity). Interacts with IRGQ (PubMed:39481378).INTERACTION Ubiquitous. Expressed at high levels in the brain, heart, prostate, ovary, spleen and skeletal muscle. Expressed at very low levels in lung, thymus and small intestine.PTM The precursor molecule is cleaved by ATG4 (ATG4A, ATG4B, ATG4C or ATG4D) to expose the glycine at the C-terminus and form the cytosolic form, GABARAPL2-I (PubMed:15169837, PubMed:20818167, PubMed:30661429, PubMed:31709703). The processed form is then activated by APG7L/ATG7, transferred to ATG3 and conjugated to phosphatidylethanolamine (PE) phospholipid to form the membrane-bound form, GABARAPL2-II (PubMed:15169837, PubMed:31709703). During non-canonical autophagy, the processed form is conjugated to phosphatidylserine (PS) phospholipid (PubMed:33909989). ATG4 proteins also mediate the delipidation of PE-conjugated forms required for GABARAPL2 recycling when autophagosomes fuse with lysosomes (PubMed:29458288, PubMed:31709703, PubMed:33909989). In addition, ATG4B and ATG4D mediate delipidation of ATG8 proteins conjugated to PS during non-canonical autophagy (PubMed:33909989). ATG4B constitutes the major protein for proteolytic activation (PubMed:30661429). ATG4D is the main enzyme for delipidation activity (By similarity).PTM (Microbial infection) The Legionella effector RavZ is a deconjugating enzyme that hydrolyzes the amide bond between the C-terminal glycine residue and an adjacent aromatic residue in ATG8 proteins conjugated to phosphatidylethanolamine (PE), producing an ATG8 protein that is resistant to reconjugation by the host machinery due to the cleavage of the reactive C-terminal glycine (PubMed:31722778). RavZ is also able to mediate delipidation of ATG8 proteins conjugated to phosphatidylserine (PS) (PubMed:33909989).PTM Phosphorylation at Ser-87 and Ser-88 by TBK1 prevents interaction with ATG4 (ATG4A, ATG4B, ATG4C or ATG4D) (PubMed:31709703). Phosphorylation by TBK1 on autophagosomes prevents their delipidation by ATG4 and premature removal from nascent autophagosomes (PubMed:31709703).SIMILARITY Belongs to the ATG8 family.
crossReference	[DatabaseIdentifier:11519034] ENSEMBL:ENSG00000034713 [DatabaseIdentifier:12466696] RefSeq:NP_009216.1 [DatabaseIdentifier:12288276] Pharos - Targets:P60520 [DatabaseIdentifier:12269643] PRO:P60520 [DatabaseIdentifier:12164072] PDB:8Q6Q [DatabaseIdentifier:12203043] PDB:7LK3 [DatabaseIdentifier:12203044] PDB:4CO7 [DatabaseIdentifier:12203042] PDB:6H8C [DatabaseIdentifier:12203041] PDB:7YO8 [DatabaseIdentifier:12128051] OpenTargets:ENSG00000034713 [DatabaseIdentifier:11843442] HPA:ENSG00000034713-GABARAPL2 [DatabaseIdentifier:11831474] HMDB Protein:HMDBP10646 [DatabaseIdentifier:11790433] GeneCards:GABARAPL2 [DatabaseIdentifier:11759872] ENSEMBL:ENST00000037243 [DatabaseIdentifier:11637173] ENSEMBL:ENSP00000037243
databaseName	UniProt
dbId	55556
description	recommendedName: fullName evidence="37"Gamma-aminobutyric acid receptor-associated protein-like 2 alternativeName: GABA(A) receptor-associated protein-like 2 alternativeName: Ganglioside expression factor 2 shortName: GEF-2 alternativeName: General protein transport factor p16 alternativeName: Golgi-associated ATPase enhancer of 16 kDa shortName: GATE-16 alternativeName: MAP1 light chain 3-related protein
displayName	UniProt:P60520 GABARAPL2
geneName	GABARAPL2 FLC3A GEF2
identifier	P60520
isSequenceChanged	false
keyword	3D-structure Acetylation Autophagy Cytoplasmic vesicle Endoplasmic reticulum Golgi apparatus Lipoprotein Membrane Phosphoprotein Protein transport Proteomics identification Reference proteome Transport
modified	[InstanceEdit:9948485] Weiser, Joel, 2025-05-21
moleculeType	Protein
name	GABARAPL2
otherIdentifier	11345 11728661_a_at 11728662_s_at 11747514_a_at 11754495_x_at 16821076 209046_PM_s_at 209046_s_at 35767_at 3669060 3669061 3669062 3669064 3669067 3669071 3669072 3669073 7997272 A_23_P118313 A_24_P356338 GE61629 GO:0000045 GO:0000139 GO:0000149 GO:0000421 GO:0000423 GO:0005515 GO:0005543 GO:0005737 GO:0005773 GO:0005776 GO:0005783 GO:0005789 GO:0005794 GO:0005829 GO:0006891 GO:0006914 GO:0006995 GO:0008017 GO:0008092 GO:0008429 GO:0015031 GO:0016020 GO:0016192 GO:0031410 GO:0031625 GO:0032781 GO:0048487 GO:0050811 GO:0051117 GO:0070972 GO:0097352 GO:1901799 HMNXSV003049457 ILMN_1796458 ILMN_2046315 PH_hs_0027060 PH_hs_0039504 TC16000620.hg g12641848_3p_s_at
physicalEntity	[EntityWithAccessionedSequence:R-HSA-5671758] GABARAPL2 [cytosol] - Homo sapiens [EntityWithAccessionedSequence:R-HSA-5682641] GABARAPL2(1-117) [cytosol] - Homo sapiens [EntityWithAccessionedSequence:R-HSA-5683633] GABARAPL2 [autophagosome membrane] - Homo sapiens
referenceDatabase	[ReferenceDatabase:2] UniProt
referenceGene	[ReferenceDNASequence:ensembl:ENSG00000034713] ENSEMBL:ENSG00000034713 GABARAPL2 [ReferenceDNASequence:12767024] UCSC:P60520 GABARAPL2 [ReferenceDNASequence:12112859] OMIM:607452 GABARAPL2 [ReferenceDNASequence:12096859] NCBI Gene:11345 GABARAPL2 [ReferenceDNASequence:12012766] Monarch:11345 GABARAPL2 [ReferenceDNASequence:11919715] KEGG:hsa:11345 GABARAPL2 [ReferenceDNASequence:11822094] HGNC:13291 GABARAPL2 [ReferenceDNASequence:11441264] dbSNP Gene:11345 GABARAPL2 [ReferenceDNASequence:11417805] CTD Gene:11345 GABARAPL2 [ReferenceDNASequence:11403638] COSMIC - genes:GABARAPL2 GABARAPL2 [ReferenceDNASequence:11382977] BioGPS Gene:11345 GABARAPL2
referenceTranscript	[ReferenceRNASequence:12648156] RefSeq:NM_007285.7 GABARAPL2
schemaClass	ReferenceGeneProduct
secondaryIdentifier	GBRL2_HUMAN O08765 Q6FG91 Q9DCP8 Q9UQF7
sequenceLength	117
species	[Species:48887] Homo sapiens
stId	uniprot:P60520
url	http://purl.uniprot.org/uniprot/P60520

Referrals

(referenceEntity)	[EntityWithAccessionedSequence:R-HSA-5671758] GABARAPL2 [cytosol] [EntityWithAccessionedSequence:R-HSA-5682641] GABARAPL2(1-117) [cytosol] [EntityWithAccessionedSequence:R-HSA-5683633] GABARAPL2 [autophagosome membrane]
(referenceSequence)	[InterChainCrosslinkedResidue:9819372] Inter-chain Crosslink via S-(glycyl)-L-cysteine (Cys-Gly) at 116 and 264 [InterChainCrosslinkedResidue:9820429] Inter-chain Crosslink via S-(glycyl)-L-cysteine (Cys-Gly) at 116 and 572
(secondReferenceSequence)	[InterChainCrosslinkedResidue:9819374] Inter-chain Crosslink via S-(glycyl)-L-cysteine (Cys-Gly) at 264 and 116 [InterChainCrosslinkedResidue:10794052] Inter-chain Crosslink via S-(glycyl)-L-cysteine (Cys-Gly) at 264 and 116 (in Homo sapiens) [InterChainCrosslinkedResidue:10968747] Inter-chain Crosslink via S-(glycyl)-L-cysteine (Cys-Gly) at 264 and 116 (in Homo sapiens) [InterChainCrosslinkedResidue:10293492] Inter-chain Crosslink via S-(glycyl)-L-cysteine (Cys-Gly) at 264 and 116 (in Homo sapiens) [InterChainCrosslinkedResidue:10060115] Inter-chain Crosslink via S-(glycyl)-L-cysteine (Cys-Gly) at 264 and 116 (in Homo sapiens) [InterChainCrosslinkedResidue:10519147] Inter-chain Crosslink via S-(glycyl)-L-cysteine (Cys-Gly) at 264 and 116 (in Homo sapiens) [InterChainCrosslinkedResidue:10405103] Inter-chain Crosslink via S-(glycyl)-L-cysteine (Cys-Gly) at 264 and 116 (in Homo sapiens) [InterChainCrosslinkedResidue:10881716] Inter-chain Crosslink via S-(glycyl)-L-cysteine (Cys-Gly) at 264 and 116 (in Homo sapiens) [InterChainCrosslinkedResidue:10616562] Inter-chain Crosslink via S-(glycyl)-L-cysteine (Cys-Gly) at 264 and 116 (in Homo sapiens) [InterChainCrosslinkedResidue:11026799] Inter-chain Crosslink via S-(glycyl)-L-cysteine (Cys-Gly) at 264 and 116 (in Homo sapiens) [InterChainCrosslinkedResidue:10701719] Inter-chain Crosslink via S-(glycyl)-L-cysteine (Cys-Gly) at 264 and 116 (in Homo sapiens) [InterChainCrosslinkedResidue:11067504] Inter-chain Crosslink via S-(glycyl)-L-cysteine (Cys-Gly) at 264 and 116 (in Homo sapiens) [InterChainCrosslinkedResidue:11119536] Inter-chain Crosslink via S-(glycyl)-L-cysteine (Cys-Gly) at 264 and 116 (in Homo sapiens) [InterChainCrosslinkedResidue:11096371] Inter-chain Crosslink via S-(glycyl)-L-cysteine (Cys-Gly) at 264 and 116 (in Homo sapiens) [InterChainCrosslinkedResidue:11119539] Inter-chain Crosslink via S-(glycyl)-L-cysteine (Cys-Gly) at 264 and 116 (in Homo sapiens) [InterChainCrosslinkedResidue:10183011] Inter-chain Crosslink via S-(glycyl)-L-cysteine (Cys-Gly) at 264 and 116 (in Homo sapiens) [InterChainCrosslinkedResidue:9820412] Inter-chain Crosslink via S-(glycyl)-L-cysteine (Cys-Gly) at 572 and 116 [InterChainCrosslinkedResidue:10968834] Inter-chain Crosslink via S-(glycyl)-L-cysteine (Cys-Gly) at 572 and 116 (in Homo sapiens) [InterChainCrosslinkedResidue:10794143] Inter-chain Crosslink via S-(glycyl)-L-cysteine (Cys-Gly) at 572 and 116 (in Homo sapiens) [InterChainCrosslinkedResidue:11026944] Inter-chain Crosslink via S-(glycyl)-L-cysteine (Cys-Gly) at 572 and 116 (in Homo sapiens) [InterChainCrosslinkedResidue:10060208] Inter-chain Crosslink via S-(glycyl)-L-cysteine (Cys-Gly) at 572 and 116 (in Homo sapiens) [InterChainCrosslinkedResidue:10881803] Inter-chain Crosslink via S-(glycyl)-L-cysteine (Cys-Gly) at 572 and 116 (in Homo sapiens) [InterChainCrosslinkedResidue:10405200] Inter-chain Crosslink via S-(glycyl)-L-cysteine (Cys-Gly) at 572 and 116 (in Homo sapiens) [InterChainCrosslinkedResidue:10701805] Inter-chain Crosslink via S-(glycyl)-L-cysteine (Cys-Gly) at 572 and 116 (in Homo sapiens) [InterChainCrosslinkedResidue:10293585] Inter-chain Crosslink via S-(glycyl)-L-cysteine (Cys-Gly) at 572 and 116 (in Homo sapiens) [InterChainCrosslinkedResidue:10519244] Inter-chain Crosslink via S-(glycyl)-L-cysteine (Cys-Gly) at 572 and 116 (in Homo sapiens) [InterChainCrosslinkedResidue:10183112] Inter-chain Crosslink via S-(glycyl)-L-cysteine (Cys-Gly) at 572 and 116 (in Homo sapiens) [InterChainCrosslinkedResidue:11096474] Inter-chain Crosslink via S-(glycyl)-L-cysteine (Cys-Gly) at 572 and 116 (in Homo sapiens) [InterChainCrosslinkedResidue:11119612] Inter-chain Crosslink via S-(glycyl)-L-cysteine (Cys-Gly) at 572 and 116 (in Homo sapiens) [InterChainCrosslinkedResidue:11067601] Inter-chain Crosslink via S-(glycyl)-L-cysteine (Cys-Gly) at 572 and 116 (in Homo sapiens)
(interactor)	[UndirectedInteraction:11304269] UniProt:Q92945 <-> UniProt:P60520 (IntAct) [UndirectedInteraction:11304091] UniProt:O00410 <-> UniProt:P60520 (IntAct) [UndirectedInteraction:11303307] UniProt:Q9UH92 <-> UniProt:P60520 (IntAct) [UndirectedInteraction:11301591] UniProt:Q14596 <-> UniProt:P60520 (IntAct) [UndirectedInteraction:11296859] UniProt:O60238 <-> UniProt:P60520 (IntAct) [UndirectedInteraction:11293241] UniProt:Q8IVP5 <-> UniProt:P60520 (IntAct) [UndirectedInteraction:11288357] UniProt:P63104 <-> UniProt:P60520 (IntAct) [UndirectedInteraction:11282307] UniProt:Q676U5 <-> UniProt:P60520 (IntAct) [UndirectedInteraction:11279529] UniProt:Q9NV35 <-> UniProt:P60520 (IntAct) [UndirectedInteraction:11277935] UniProt:Q96A56 <-> UniProt:P60520 (IntAct) [UndirectedInteraction:11268671] UniProt:P15880 <-> UniProt:P60520 (IntAct) [UndirectedInteraction:11268455] UniProt:P46934 <-> UniProt:P60520 (IntAct) [UndirectedInteraction:11255591] UniProt:Q13685 <-> UniProt:P60520 (IntAct) [UndirectedInteraction:11244403] UniProt:Q9Z2F7 <-> UniProt:P60520 (IntAct) [UndirectedInteraction:11241783] UniProt:Q8TC07 <-> UniProt:P60520 (IntAct) [UndirectedInteraction:11134599] UniProt:Q13188 <-> UniProt:P60520 (IntAct) [UndirectedInteraction:11135381] UniProt:Q9NT62 <-> UniProt:P60520 (IntAct) [UndirectedInteraction:11140009] UniProt:Q13501 <-> UniProt:P60520 (IntAct) [UndirectedInteraction:11184601] UniProt:P07237 <-> UniProt:P60520 (IntAct) [UndirectedInteraction:11184981] UniProt:Q8N6T3 <-> UniProt:P60520 (IntAct) [UndirectedInteraction:11194123] UniProt:Q13043 <-> UniProt:P60520 (IntAct) [UndirectedInteraction:11203693] UniProt:Q9GZQ8 <-> UniProt:P60520 (IntAct) [UndirectedInteraction:11233727] UniProt:Q3MII6 <-> UniProt:P60520 (IntAct) [UndirectedInteraction:11234019] UniProt:P22061 <-> UniProt:P60520 (IntAct) [UndirectedInteraction:11234213] UniProt:P00533 <-> UniProt:P60520 (IntAct) [UndirectedInteraction:11241359] UniProt:O75385 <-> UniProt:P60520 (IntAct)

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